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Chinese Journal of Clinical Anatomy
(Founded in July 1983 Bimonthly)
Responsible Institution: China Association for Science and Technology
Sponsor: Chinese Society of Anatomical Sciences
Editing and Publishing: Editorial Board of Chinese Journal of Clinical Anatomy
Advisor: ZHONG Shi-zhen
Editor-in-chief: OUYANG Jun
Former Editor-in-chief: XU Da-chuan
Overseas Distributor: China International Book Trading Corporation (P.O. Box 399, Beijing, China, Code No. BM5961)
Address: 1023 Shatainan Road, Guangzhou, 510515, China
Tel: (020)61648203
E-mail: journal@chjcana.com
CN: 44-1153/R
ISSN: 1001-165X

Table of Content

    25 March 2018 Volume 36 Issue 2
      
    Localization and significance of the nerve entry points and the center of the highest region of the muscle spindle abundance in the lateral muscle group of leg
    ZHUO Li-fan,HU Shuai-yu, PAN Yan-mei, KE Zhi-teng, YANG Sheng-bo
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  121-126.  doi:10.13418/j.issn.1001-165x.2018.02.001
    Abstract ( 916 )  
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    Objective To localize the location of the nerve entry points (NEPs) and the center of the highest region of the muscle spindle abundance (CHRMSA) in the lateral muscle group  of the leg. Methods Twelve adult cadavers were placed in a side-lying position. The curved lines on skin surface joining the lateral epicondyle and the medial epicondyle of the femur and the lateral epicondyle of the femur and lateral malleolus of the fibula were respectively defined as the horizontal reference line (H) and the longitudinal reference line (L). Cadavers were dissected to expose NEPs; Sihler 's staining was adopted for showing the intramuscular nerve dense regions; HE staining was performed for demonstrate muscle spindles in order to calculate their abundance; barium sulfate was used to label the NEPs and CHRMSAs, and then CT scan. The body surface projection point of the NEP was designated as P, whereas the projection in the opposite direction across the transverse plane was designated as P'. The intersection between the vertical line and line H, and that between the horizontal line and line L through P were designated as PH and PL, respectively. The percentage location of PH and PL on the line H and line L and depth of NEP were determined. Results The PH and PL  of the NEP of peroneus longus and peroneus brevis muscles were located at 13.14% and 10.35% of line H and 21.81% and 52.6% of line L, respectively. The depth of NEP was at 50.89% and 25.7% of line PP', respectively. The PH of the CHRMSA of the peroneus longus and peroneus brevis muscles were located at 14.45% and 12.86% of the line H and the PL is at 35.11% and 71.49% of the line L, respectively. The depth were at 18.16% and 20.40% of the line PP', respectively. Conclusion These results should provide an anatomical guidance for accurately localizing the block targets in treatment of spasticity in the lateral muscles.

    The study of the nerve and vascular anatomy in the bifurcation region of the carotid artery and its clinical significance in carotid artery intimal dissection
    TAN Qing-jing, QIN Chao, YANG Jun-wei,WANG Tian-bao, LIN Hao-hai,ZHANG De-min, JIANG Zhi-bin
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  127-131.  doi:10.13418/j.issn.1001-165x.2018.02.002
    Abstract ( 887 )  
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    Objective To study the nerve and blood vessels in the carotid bifurcation area in cadavers so that the morphological diagnostic basis and anatomic basis could be provided for safe and effective performance of carotid endarterectomy. Methods 20 cases (40 sides) of autopsy specimens with no clear heart and cerebrovascular diseases before death were selected in our school (male 11 cases, female 9 cases). The anatomic study on the nerve and blood vessels in the carotid bifurcation area was performed using the surgical approach of carotid endarterectomy, and morphological and structural features of blood vessels and the relationship between the nerve and the blood vessels were explored. Results The types of common carotid artery bifurcation: type I accounted for 10%; type II accounted for 52.5%; type III accounted for 37.5%. The height of the bifurcation of the common carotid artery: above the top of the thyroid cartilage: 55% on the left side and 62.5% on the right; parallel to upper edge of thyroid cartilage on the upper edge: 37.5% on the left side of the, and the 27.5% on right side of the; below the upper edge of the thyroid cartilage: 7.5% on the left side, and 10% on the right side. The internal diameter of common, internal and external carotid arteries of males were wider than that of females(P<0.05), so was the bilateral carotid bifurcation angle(P<0.05). No statistically significant correlation was found between the internal diameters of common, internal and external carotid arteries of left side and right side. The bilateral carotid bifurcation angle of males was larger than that of female(P<0.05). Conclusion The anatomical measurement data of nerves and vessels on the carotid bifurcation region can not only define the relationship between neck blood vessels and nerves, but also provide morphological evidence and anatomic basis for safe and effective implementation of carotid endarterectomy.

    Anatomic classification of the inferior angle of scapula and its clinical significance
    ZHANG Lei, QIN Bo, GUO Xiao-guang, QI Ji, XU Yan-xiao, FU Shi-jie
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  132-136.  doi:10.13418/j.issn.1001-165x.2018.02.003
    Abstract ( 2504 )  
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    Objective To study the morphology of the inferior angle of scapula, and to explore its clinical significance. Methods The inferior angle of scapulae were measured in 275 dry scapular bones. Reference points: a: inferior angle; b: inferior glenoid tubercle; c: the intersection of the medial edge of the scapula and the scapular spine; e: scapular angle; f: spinoglenoid notch; d: the intersection of the line cf and the vertical line through point a. The parameters of inferior angles of 275 dry scapular bones, including the thickness of point a (the thickest part of inferior angle of the scapula), the length of the line ab, line ac, and line ad, and the angle of ∠bac, ∠ace (the projection of the scapular sagittal plane), and ∠ace′(the projection of the scapular coronal plane), were observed and measured using Vernier caliper and Goniometer. Results The morphology of the inferior angle of scapulae can be divided into 3 types, namely, Accessory angle-type (145, 52.73%), U-type (87, 31.64%) and V-type (43, 15.64%). In terms of the thickness of point a and the length of line ab, there were significant differences between the Accessory angle-type and V-type (P<0.05). With regard to the length of line ac, Accessory angle-type revealed a significant difference compared with U-type (P<0.05), or V-type (P<0.05). In terms of the length of line ad, there was a significant difference between the Accessory angle-type and V-type (P<0.05); Meanwhile, a statistical difference was found in U-type and V-type (P<0.05). With respect to ∠ace′, there was a statistical difference between Accessory angle-type and U-type (P<0.05). Besides, regarding ∠bac, the inferior angle of scapular of the right side was statistically different from that of the left side (P<0.05). Conclusion The inferior angle of scapulae have three anatomical types, with the Accessory angle-type as the main type, and the morphology and classification of it are of certain clinical implication.

    A abnormal case of right subclavian artery and right common carotid artery
    XIONG Yin-ting, ZHANG Meng-yang, XIAO Yue, TAN Guo-jing,YU Shan-shan, LI Wen-qi
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  136.  doi:10.13418/j.issn.1001-165x.2018.02.025
    Abstract ( 573 )  
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    Accuracy of patient-specific drill template cervical pedicle screws insertion via cortical bone trajectory
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  137-140.  doi:10.13418/j.issn.1001-165x.2018.02.004
    Abstract ( 452 )  
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    Objective To validate the security and accuracy of cervical pedicle screws insertion (CPSI) with the assistance of patient-specific drill templates (PDTs) via cortical bone trajectory. Methods  Four normal cervical spine specimens(C3~7) were selected and randomly divided into two groups. All specimens received CT scan. After images processing, the individual guide templates with cortical bone trajectory (CBT) were designed and subsequently produced by rapid prototyping (RP) and computer numerical control (CNC) technology, respectively. Then, their security and accuracy were evaluated in facilitating CPSI. Results The absolute deviations at the entry point were (0.28±0.24) mm in group RP and (0.32±0.23) mm in group CNC on the transverse plane, and were (0.30±0.20) mm in group RP and (0.52±0.44) mm in group CNC on the sagittal plane. The absolute deviations at the midpoint of the pedicle were (0.26±0.22) mm in group RP and (0.39±0.32) mm in group CNC on the transverse plane, and were (0.37±0.29) mm in group RP and (0.49±0.50) mm in group CNC on the sagittal plane. For the screw position, 20 screws position were in grade 1 in group RP; 19 screws position were in grade 1 and 1 was in grade 2 in group CNC. No statistically significant difference existed (P>0.05) in deviation and screw position between the two groups. Conclusion CNC-PDTs via cortical bone trajectory are viable in facilitating CPSI with good feasibility and accuracy. It is a new alternative method to lower cervical pedicle insertion.

    Sectional and applied anatomy of ventral pons operation through sphenoid-skull area
    QIAO Li-ying, ZHENG Zi-cheng, MU Bo-kun, YU Yong, WANG Ping, WU Yue
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  141-146.  doi:10.13418/j.issn.1001-165x.2018.02.005
    Abstract ( 659 )  
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    Objective To provide the evidence of applied anatomy for creating a new operative route about ventral of pons through sphenoid-skull area with improved celloidin embedding technique. Methods  Firstly,we obtained the anterior skull bases from 30 adult head specimens fixed by Faure Marin. Then, we made three-dimensional continued thin sections of 0.25 mm after series of processes including decalcification and dehydration. Besides, we observed and measured 10 decalcification heads of adult cadaver,100 sides dry bone skull specimens and 50 specimens of MRI. Results The sphenoid sinuses were various in morphology and could be wedge-like, elliptical, rectangular, broad-bean-shaped, saddle-shaped and “λ” -shaped. The sphenoid sinuses could be divided into 4 types according to the bulged optic canal, including no-bulge-type, hill-like-bulge-type, half-canal-bulge-type and whole-canal-bulge-type. The distance from anterior to posterior wall of sphenoid sinus was(10.7±5.8)mm on the left, and(14.0±4.9)mm on the right. The MRI median sagittal plane of posterior wall of the sphenoid sinus type Ⅰ(posterior wall of a membrane like structure or thin bone plate) accounted for 64%; posterior wall of sphenoid sinus type Ⅱ (posterior wall as a part of the slope) accounted for 36%. Conclusion With the new approach of the operation, the nasal endoscope can go into sphenoid sinuses through the middle nasal meatus. Then it can pass through the posterior walls of sphenoid sinus vertically and have an operation on ventral of pons. Our outcome will supply reliable anatomical evidence for the new route.

    Application of head CT 3D-reconstruction for operation planning of nasal inverted papilloma
    CAI Li,MA Jian-gang,YU Lei, MAO Yi-nan,ZHOU Kun-peng,YANG Yan-zhong, REN Guo-shan
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  147-152.  doi:10.13418/j.issn.1001-165x.2018.02.006
    Abstract ( 655 )  
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    Objective To use the technology of three-dimensional (3D)reconstruction based on head CT for  preoperation  planning and postoperative  assessment in the nasal inverted papilloma surgery, and to provide reference value for clinical application. Method This study selected a male patient, who was 65 years old with a weight of 70 kg and height of 170 cm, and had  no head trauma history, surgery history and family history of brain disease. Head CT scans were performed to exclude organic brain diseases. Informed consent has been signed with the patient. The reverse engineering software Mimics 15.0 was used to create a 3D-reconstruction model, the model components were measured by 3-matic 7.0, and the preoperative planning and follow-up observation for the nasal inverted papilloma operation were designed. Result The 3D-models of head skull and sinus were established in accordance with the CT image measurement results;  a 3D model of simulated nasal passages and tumors that consistent with the surgical approach was established; the surgical design of complicated nasal tumor and assessment after eradication were further completed. Postoperative tumor measurements showed a maximum diameter of 64.78 mm on the X-axis, 71.21 mm on the Y-axis, 86.46 mm on the Z-axis, and a total volume of 58.88 ml. This was in accord with the preoperative measurement results in shape and size. According to the analysis, the size of the tumor tissue resected during the operation was basically the same as the size of the preoperative 3D-model reconstructed tumor, indicating that the tumor tissue was completely resected by the operation. Conclusion Through the data analyzed by the 3D model,  we can design pre-operation  planning and complete the operation successfully. It is of great significance to guide the clinical design of individualized surgical approach for sinus tumors.

    The anatomic study of cerebral cortical and medullary veins on susceptibility-weighted imaging in acute ischemic stroke
    ZHANG Xiao-fen, WEN Cai-Yun, LI Jian-Ce, CHEN Xiao-Li, CHEN Zheng-Zhen, CHEN Cheng-Chun
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  153-157.  doi:10.13418/j.issn.1001-165x.2018.02.007
    Abstract ( 360 )  
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    Objective To assess the features of the cortical and medullary veins on the susceptibility weighted imaging(SWI) in patients with hyperacute and acute ischemic stroke and its relationship with stroke severity and the anatomical characteristics of the infarctions to provide the clinical diagnosis information and treatment related reference and guidance for patients with stroke.    Methods    We selected 38 stroke patients hyperacute ischemic and 62 patients with acute ischemic stroke. The examinations were conducted using MRI. The stroke severity and anatomy of infracts were assessed. Results The NIHSS score was 8(4~8.8)in PAVS(+) and 5.5(3~8)in PAVS(-) for hyperacute ischemic stroke (P=0.058). The NIHSS score was 5 (2~7) in PAVS(+) and 1.5 (1~2) in PAVS(-) for acute stroke (P=0.043). The frequency of PAVS was 82.8% in the anterior circulation ischemic stroke and 28.6% in the posterior circulation ischemic stroke (P<0.001).   Conclusion The appearance of PAVS represents poorer stroke severity in the acute ischemic stroke, except for the hyperacute ischemic stroke. The anterior circulation acute stroke tends to represent PAVS.

    The relationship between bifurcation angle and main trunk length and atherosclerosis of left coronary artery in Uighur and Han
    LIU Xiao-bei, PAN Cun-xue, LIU Wen-ya
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  158-161.  doi:10.13418/j.issn.1001-165x.2018.02.008
    Abstract ( 394 )  
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    Objective To analyzed the relationship between the bifurcation angle and the length of the main trunk and the atherosclerosis of the left Coronary artery in Uighur and Han by MSCT. Methods   Coronary artery CTA imaging data of six hospitals from 2013.6 to 2014.12 was retrospectively analyzed. Patients were classified asⅠ~Ⅳ types according to the degree of luminal stenosis of at the left coronary bifurcation region. Comparison the different types of the bifurcation angle, and the relationship between the bifurcation angle and the length of the main trunk of the left Coronary artery was performed. Results The study enrolled 1276 cases, in which 916 cases were of Han ethnicity, and 360 cases of Uighur ethinicity. The bifurcation angle of Han was (84±38.8)º , with a range of (29º~158º); the length of the main trunk was (9.2±5.3) mm, with a range (1.6~28.6 mm); In Uighur the bifurcation angle was (85±34)º, with a range (24º~155º); the length of main trunk length was (9.6±36.6) mm, with a range (2.4~18.3 mm). There was significant difference amongⅠand Ⅱ, Ⅲ and Ⅳ types and no significant difference among Ⅱ, Ⅲ or Ⅳ types in either Han or Uighur ethnicity. There was moderate correlation between the bifurcation angle and the length of the main trunk of the left coronary artery (Han R= 0.307; Uighur R=0.421). Conclusion A larger left coronary bifurcation angle and a greater length of the left main trunk are more likely to cause coronary artery atherosclerosis in the bifurcation region, but there is no significant correlation with the degree of luminal stenosis. The left coronary bifurcation angle is moderately related to the length of the main trunk of the left coronary artery, and the Uighur is slightly higher than Han.

    CT anatomic study of position correlation between descending colon and left kidney
    WENG Ai-ting, TU Ying-shan, WANG Qian-qian, GE Yan-ming, SHAO Wei-guang, DONG Peng
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  162-164.  doi:10.13418/j.issn.1001-165x.2018.02.009
    Abstract ( 1043 )  
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    Objective To discuss the correlation between subarea of the descending colon relative to the left kidney (SaDCK) and the type of anatomic position of the left lateral conal fascia formed by the anterior and posterior renal fasciae outside of the left kidney (APLCF) using multi-slice spiral computed tomography. Methods  The abdominal CT imaging data of 121 patients were collected and analyzed retrospectively. The SaDCK and the type of the APLCF were studied, and the corresponding value was recorded.  Results (1) 72 patients showed that the type of the left APLCF belonged to type I, and 49 patients showed that the type of the left APLCF belonged to type II; (2) The anatomic position of descending colon showed a trend from the front to the posterolateral side of the left kidney; (3) There was a correlation between the SaDCK and the left APLCF at the level of the renal hilum (χ2=6.254, P<0.05). Conclusion  There was a correlation between the SaDCK and the type of left APLCF.

    Effects of low magtitude and high frequency vibration on the biological characteristics of osteoblasts
    ZHA Ding-sheng, DENG Xuan-geng, CHEN Jian-ting
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  165-173.  doi:10.13418/j.issn.1001-165x.2018.02.010
    Abstract ( 485 )  
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    Objective To study the effects of low-magnitude high-frequency vibration (LMHFV) on the biological characteristics of osteoblasts. Methods To establish a model of MC3T3-E1 cells loaded with LMHFV, and observe the effects of LMHFV at different frequencies on the concentration ratio of OPG / RANKL in MC3T3-E1 cells, the frequency with the highest OPG / RANKL concentration ratio (F) was the frequency of follow-up studies. With 0 Hz as a control, to observe the effects of LMHFV on the expression of akialine phosphatase (ALP), osteocalcin (OCN) mRNA and protein activity, and calcified nodules in MC3T3-E1 cells; RAW264.7 cells were incubated with conditioned medium (CMF) loaded with LMHFV, and to observe the effect of CMF on TRAP staining, multinucleated osteoclast formation, TRAP mRNA and protein activity in osteoclasts; to study the expression of cyclooxygenase-2 (COX-2) protein level in MC3T3-E1 cells loaded with LMHFV and the effect of COX-2 inhibitor NS-398 on the differentiation of MC3T3-E1 cells induced by LMHFV. Results The highest concentration ratio of OPG / RANKL was obtained at 30 Hz in LMHFV, which promoted the increase of ALP, OCN mRNA and protein activity and increased the formation of calcified nodules. CM formed by LMHFV at 30 Hz inhibited the differentiation of RAW264.7 cells into multinucleated osteoclasts and inhibited the TRAP mRNA and protein activity. LMHFV induced an increase in COX-2 protein level and NS-398 inhibited LMHFV-induced osteoblast differentiation. Conclusion LMHFV at 30 Hz had a positive effect on OPG / RANKL concentration ratio and osteogenic differentiation in MC3T3-E1 cells, indirectly suppresses bone resorption by regulating the osteoblast OPG / RANKL concentration ratio, the COX-2 pathway is involved in the regulation of osteoblast biological properties by LMHFV.

    Production of decellularized porcine, leporine and murine renal biological scaffold and co-culture exploration of renal scaffolds slices with cells in vitro
    WANG Xin-wang,ZHOU Le-bin,MEI Jin,ZHANG De-ming,LIU Dan,DU Ao-ling,CHEN Sheng-hua
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  174-181.  doi:10.13418/j.issn.1001-165x.2018.02.011
    Abstract ( 402 )  
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    Objective To produce the decellularized biological renal scaffold of the pig, New Zealand white rabbit and SD rat, and then to explore the influence of porcine, leporine and murine renal biological scaffolds on the co-cultured seed cells HEK. Methods Adult healthy kidneys from 10 pigs, 28 New Zealand white rabbits, and 28 SD rats were harvested and divided into normal groups and scaffold groups randomly and equally. The scaffold group kidneys were perfused for decellularization with heparinized PBS, 1% Triton X-100 and 1% SDS through the renal artery successively. The kidneys in each group were characterized in histomorphology and detected for mechanical properties. And the co-culture experiments were conducted using the slices of each group and HEK cells, and the expression of PCNA in HEK cells was analyzed by immunofluorescence assay using the value of the gray level. Results In each scaffold group after decellularization, the HE staining of cell nucleus in the slices was negative, Masson's trichrome staining of collagen was positive, and immunofluorescence staining of Collagen I and Collagen IV was also positive. And with the scanning electron microscopy, the faveolate orifice structure and the typical glomerulus-like structure were observed. Characterized by the mechanical properties, the Young's modulus of each scaffold group is similar with that of the corresponding normal group. After the co-culture, the PCNA/DAPI value of every renal scaffold group is higher than that of the blank control. Furthermore, there was no significant difference on the PCNA/DAPI of HEK between the pig, New Zealand white rabbit and SD rat renal scaffolds.  Conclusions The decellularization method of our study can eliminate the cells in the porcine, leporine and murine kidney, retain the extracellular matrices and preserve the spatial structure and similar mechanical strength. And it is an effective way to produce the decellularized pig, New Zealand white rabbit and SD rat renal biological scaffolds. Importantly, the produced decellularized porcine, leporine and murine kidney scaffold slices can improve the proliferation activity of heterogeneous HEK cells when co-cultured in vitro, and there is no species difference on the improvement between the pig, New Zealand white rabbit and SD rat.

    Correlation between the degree of cerebral ischemia-reperfusion injury and the expression of LC3 and mTOR
    LI Dan-dan,XIAO Xue-jin,QIN Shu-jian,BAO Cui-fen
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  182-186.  doi:10.13418/j.issn.1001-165x.2018.02.012
    Abstract ( 466 )  
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    Objective To investigate the correlation between the degree of focal cerebral ischemia-reperfusion injury and the expression of LC3-Ⅱ and mTOR. Methods The models of focal cerebral ischemia-reperfusion in rats were established by occlusion of the middle cerebral artery. Neurological deficit score and TTC infarct volume were observed among groups. The expressions of LC3-Ⅱ and mTOR in different groups were detected by immunoblotting and immunofluorescence.   Results Immunoblotting and immunofluorescence showed that LC3-Ⅱ had the highest expression in 24 hours after ischemia and reperfusion (IR 24 h), and mTOR was the lowest in IR 24h and IR 48 h. Correlation analysis showed that the infarcted volume of TTC was positively correlated with the expression level of LC3-II. Conclusion  IR may activate the autophagy of the parietal neurons of the brain by increasing the expression level of LC3-II and decreasing the expression level of mTOR. The degree of cerebral ischemia-reperfusion injury may be related to the expression level of LC3 and mTOR.

    Osteogenic differentiation of rat bone marrow mesenchymal stem cells induced by bone morphogenetic protein gene 2 vector
    GAO Jun-huai, QIU Su-jun, YIN Jie, SHI Ben-chao, MIN Shao-xiong
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  187-191.  doi:10.13418/j.issn.1001-165x.2018.02.013
    Abstract ( 492 )  
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    Objective To study the effect of acetylcysteine/phosphorylcholine-modified chitosan (NAC-PCCs) encapsulation of bone morphogenetic protein gene 2 nanoparticle materials in promoting the osteogenic differentiation of SD rat bone marrow mesenchymal stem cells (BMSC) in vitro. Methods The pBMP2/NAC-PCCs nanoparticles were prepared, and its characterization, in vitro release and the ability to protect bone morphogenetic protein gene 2 against DNaseⅠwere examined. BMSC cells, nanoparticles in the process of cultivation, and transfection efficiency were determined by fluorescence microscopy and flow cytometry. The secretion of BMP2 protein levels was determined by ELISA experiment and the expression of RUNX2 and OC gene was determined by RT-PCR. The calcium deposition analysis was performed using alizarin red staining. Results Gel electrophoresis showed that nanoparticles could effectively protect DNA from being digested by DNaseⅠ. Fluorescent microscopy and flow cytometry showed that the gene delivery nanoparticles had better transfection efficiency. The results of ROS activity test showed that the material had the ability to scavenge ROS, and the ELISA experiment showed that the expression level of BMP2 in the group of pBMP2/NAC-PCCs was the highest within 14 days. QRT-PCR, AKP activity detection and alizarin red staining showed that the osteogenic differentiation of pBMP2/NAC-PCC was the best. Conclusion pBMP2/NAC-PCCs nanoparticles can promote the osteogenic differentiation of BMSC.

    Regulation of JNK/ERK signal pathway on Activin A mediated the recombination of F-actin of fibroblasts
    CHEN Xiao-feng, ZHANG Min, WU Wei, ZHANG Lin
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  192-196.  doi:10.13418/j.issn.1001-165x.2018.02.014
    Abstract ( 686 )  
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    Objective To explore whether Activin A regulate the filamentous actin of fibroblasts via MAPK signaling pathway. Methods Fibroblasts were Isolated and cultured from C57BL/6 rat skin which was born within 24 hours. The experiments were divided into PBS group, Activin A group, JNK specific inhibitor SP600125 group, ERK specific inhibitor SL327 group and p38 specific inhibitor SB202190 group. When cells were passaged for 3~5 generations, the total protein of cells was extracted, and the phosphorylation activity of JNK, ERK and p38 was detected by western blot. The changes of F-actin were observed by Phallotoxins staining. Results Activin A could induce the accumulation of F-actin in fibroblasts. After the use of JNK specific inhibitor SP600125, the F-actin aggregation of fibroblasts was completely inhibited. When the ERK specific inhibitor SL327 was used, the fibroblast F-actin aggregation was weakened, but not yet completely inhibited while P38 specific inhibitor SB202190 did not suppress the fibroblast F-actin aggregation. Conclusion  Activin A can induce the F-actin recombination of fibroblasts via JNK and ERK signaling pathway.

    Periodontal disease in pregnancy rats induced insulin resistance in adipose cell of adult offspring
    SHI Yang,YU Hong-yu
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  197-201.  doi:10.13418/j.issn.1001-165x.2018.02.015
    Abstract ( 503 )  
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    Objective    To explore the relationship between maternal periodontal disease and PLBW and whether the offspring of rats with periodontal disease develop insulin resistance in adulthood. Methods  12 female Wistar rats were divided into Periodontal disease (PD) and control (CN) groups, with 6 rats in each group. The rats in two groups were mated at the seventh day after induction of Periodontal disease. After delivery, male offspring were divided into Periodontal disease offspring (PDO) and control offspring (CNO), with 18 rats each group. Weight of the newborns was measured from birth to eleventh week. 11 weeks later, all offspring rats were euthanized and the glycemia, insulinemia, adiponectin content, level of TNF-α, Akt serine phosphorylation levels and Glut4 content in the adipose tissue were measured.  Results Weight of birth, weekly body weight and glucose content had no difference between CNO and PDO groups (P>0.05). However, the concentrations of insulin, adiponectin and TNF-α were increased in PDO group compared to CNO group. Akt serine phosphorylation levels increased in both groups after insulin stimulation (P<0.05), but PDO group increased less than CNO group(P<0.05). Expression level of Glut4 in the adipose tissue decreased in PDO group compared to CNO group(P<0.05). Conclusion There is no relationship between maternal periodontal disease and PLBW, but periodontal disease during pregnancy can induce insulin resistance in adipose cell of adult offspring.

    Expression and clinical significance of XKRX in colorectal cancer
    PAN Yang-jian,GAO yuan,LIU Xiao-long, SUN Jing-bo, HUANG Xiao-ping, LIU Li-xin
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  202-205.  doi:10.13418/j.issn.1001-165x.2018.02.016
    Abstract ( 754 )  
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    Objective To explore the expression and clinical significance of XK related, X-linked (XKRX) in colorectal cancer tissue. Methods The gene expression of XKRX and corresponding clinical data in colorectal cancer in TCGA public database were collected to make retrospective analysis and survival analysis. Quantitative real-time PCR and western blot were conducted to assess the expression of XKRX in 21 cases of colorectal cancer tissues. Results We found that the expression level of XKRX in colorectal cancer tissues was significantly higher than that of adjacent tissues (P<0.0001). The mRNA and protein expression level of XKRX in colorectal cancer tissues were significantly higher than that of the tissues adjacent to carcinoma. There was no significant difference between XKRX expression and gender (P= 0.9034), age (P=0.886), TMN stage (P=0.3979), lymph node metastasis (P=0.7995) and distant metastasis (P=0.6032). However, the survival time of the group with XKRX high expression was significantly lower than that of the group with low expression (P=0.0075). Conclusion Our data shows that XKRX is up-regulated in colorectal cancer tissues and may play a role in the proto-oncogene, which may affect the malignant progression of colorectal cancer and reduce the survival time of patients.

    Screening of drug concentration of resveratrol in POI rats
    LI Ning,LIU Li-li
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  206-210.  doi:10.13418/j.issn.1001-165x.2018.02.017
    Abstract ( 756 )  
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    Objective To observe the curative effect of different concentrations of resveratrol on rats with premature ovarian insufficiency (POI) and to determine the optimal drug concentration of resveratrol for treating premature ovarian insufficiency dysfunction in rats. Methods A total of 90 5-week-old SPF grade SD healthy female rats were randomly divided into a low-dose resveratrol group (18 rats in group a) and a middle-dose resveratrol group (18 rats in group b), a high dose resveratrol group (18 rats in group c), a model group (18 rats in group d) and a blank control group (18 rats in group e). Group a, group b, group c were administered with a daily dose of 20 mg/kg, 50 mg/kg, 80 mg/kg by gavage, respectively; the model control group was administered with 0.5% sodium carboxymethyl cellulose solution by gavage. ELISA was used to detect the level of serum sex hormone levels: anti-Mullerian hormone (AMH), luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E2) level and HE staining of rats to observe unit ovarian area of mature follicles, atresia follicles and corpus luteum, evaluation of resveratrol efficacy. Results Rats treated with different concentration of resveratrol had a significantly increased level of serum AMH and E2 compared to rats treated with 0.5% carboxymethyl cellulose solution (P<0.05), LH and FSH levels were lower (P<0.05).The serum level of AMH and E2 in POI rats treated with resveratrol at different concentration were lower (P<0.05) and LH and FSH were higher (P<0.05) than those of rats in the blank control. Compared with rats in group a, b, c and e, the number of granulosa cells, mature follicles and corpus luteum in ovaries was smaller in group d (P<0.05); In contrast, the number of atresia follicles was significantly increased in group d(P<0.05).  Conclusion Intragastric treatment with different concentration of resveratrol of all have a certain effect on POI rats receiving, with 50 mg/kg showing the best result, and thus being the best drug concentration.

    A case report about variation of the subphrenic artery and the left accessory renal artery were common origin from the left renal artery
    Qin An-qi, Ma Jian-jun, Shi Chuan-kai, Liu Fang-yuan, Duan Ke-feng, Zhang Da, Zhou Jing-yi, Tan Yi-heng
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  210.  doi:10.13418/j.issn.1001-165x.2018.02.026
    Abstract ( 781 )  
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    Biomechanical comparison of Lisfranc ligament reconstruction with suture endobutton and plantaris tendon
    XU Shi-ming,CAI Hui,DENG Zi-wen,GUO Chun-li,SUN Da-wei,ZHUO Ri-bo, HUANG Dong
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  211-215.  doi:10.13418/j.issn.1001-165x.2018.02.018
    Abstract ( 519 )  
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    Objective Biomechanical comparison of Lisfranc ligament reconstruction with suture endobutton and plantaris tendon was conducted to evaluate the effect of the two methods on restoring the stability of Lisfranc joint and to provide a theoretical basis for clinical treatment of Lisfranc's injury. Methods Ten fresh adult cadaveric calves were selected and the complete Lisfranc ligament model, the injury model, the suture endobutton and plantar tendon reconstruction model were prepared. The axial or abduction load was recorded respectively. The average displacement of the basement of  the medial cuneiform (C1) to the second metatarsal (M2) changes was recorded. Results The average displacement of C1~M2 under axial load and abduction load was (0.70±0.05) mm and (1.21±0.10) mm in the intact model, (1.59±0.07) mm and (3.73±0.11) mm in the damage model,  (0.84±0.04) mm and (1.29±0.06) mm in the reconstruction model of suture endobutton, (1.01±0.05) mm and (1.34±0.05) mm in the plantar tendon reconstruction model, respectively. The displacement of the intact model and the reconstruction model C1~M2 under axial or abduction load was significantly less than that of the injury model (P<0.05); The displacement of the reconstruction model was slightly larger than that of the intact model with no significant difference (P> 0.05). There was no significant difference in the displacement between the suture endobutton and plantar tendon reconstruction model (P>0.05); The C1~M2 displacement changes under the abduction load was more than those under the axial load in any model. Conclusions (1)The biomechanical strength provided by suture endobutton and plantar tendon reconstruction is similar in the regard of the restoration of Lisfranc joint stability. (2)Compared to changes in displacement under the axial load, the difference of experimental results under the abduction load is more obvious. Hence, it is recommended to use the abduction load Lisfranc joint mechanics test.

    Biomechanical comparison and analysis of three kinds of fixators in the treatment of Lisfranc injuries
    LIU Shuai, XU Yong-qing, ZHANG Xu-lin, SUN Pei-dong, LIANG Dong-zhu
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  215-220.  doi:10.13418/j.issn.1001-165x.2018.02.019
    Abstract ( 565 )  
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    Objective To evaluate the stability of new Ni-Ti memory alloy four-corner fixator in treatment of Lisfranc injuries and conduct comparative analysis with dorsal plate and transarticular screw fixation. Methods 6 sides of lower limbs from fresh adult cadavers were chosen to test static rigidity under the condition of 600Naxial load by ElectroForce® 3510 system. Groups were set : (A) Control, Undamaged group ; (B) Damaged group without treatment ; (C) Damaged group fixed by new Ni-Ti memory alloy four-corner fixator ;  (D) Damaged group fixed by dorsal Plate ; (E) Damaged group fixed by transarticular screw fixation. Group B , C and D were cyclically loaded at 5 Hz between 200 and 600 N for 3000 cycles to test the change of immediate stabilization and fatigue stabilization. Results Paired t test was applied to analyze the difference of each group themselves after fatigue , which turned out to be of no statistically significance (P>0.05) . Integral rigidity  in group B was less than that in group A (P<0.05) . There was no significant difference among Group A , C and D (P>0.05) , but their integral rigidity were less than that of group E (P<0.05). Conclusion New Ni-Ti memory alloy four-corner fixator could provide significant fixation strength for the treatment of Lisfranc injuries , which lays the foundation for its clinical use.

    The observation and clinical significance of redundant nerve roots of cauda equina in lumbar spinal stenosis
    REN Tao, ZHENG Ming-hui, WANG Xiang, FENG Shi-feng,QU Dong-bin
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  221-225.  doi:10.13418/j.issn.1001-165x.2018.02.020
    Abstract ( 2349 )  
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    Objective To observe the morphology of Redundant Nerve Roots(RNRs)of cauda equina in lumbar spinal stenosis and to discuss the clinical significance of RNRs. Methods From January to November 2016, 134 patients with single segmental lumbar spinal stenosis were treated in our hospital and received routine MRI scan. Based on sagittal MRI, cauda equina can be divided into RNRs(+)and RNRs(-). The median sagittal diameter, the effective sagittal diameter and the effective cross-sectional canal area of the stenotic segment of spinal canal were measured. The relationship between RNRs(+)and the degree of stenosis was analyzed statistically.  Results Among 134 patients , 61 males and 73 females, aged 24~80 years (mean age 54.4±13.6 years), RNRs was positive  in 43 cases, and negative in 91 cases. The RNRs(+) morphology can be divided into three types: "stripe" type in 29 cases (67.44%, 29/43), "grape beads" type in 6 cases (13.95%, 6/43), and "serpentine "type in 8 cases (18.60%, 8 / 43).The effective cross-sectional canal area in RNRs(+) group was significantly smaller than that in RNRs(-)(P<0.05), but no significant difference was found between median sagittal diameter and effective sagittal diameter in both groups (P>0.05) .  Conclusion  RNRs of cauda equina can be found in severe lumbar spinal stenosis and it is associated with absolute stenosis of the spinal canal. Therefore, it is suggested RNRs can be used as an indicator for surgical treatment of lumbar spinal stenosis.

    The Saphenous neurocutaneous vascular flap in the clinical application of diabetic foot ulcers
    YANG Ya-dong, ZHOU Juan, TANG Wen,ZHANG Fei, LIU Yang,WEI Xiao-jun
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  226-230.  doi:10.13418/j.issn.1001-165x.2018.02.021
    Abstract ( 331 )  
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    Objective To investigate the clinical efficacy of the saphenous neurocutaneous vascular flap in diabetic foot ulcers. Methods From September 2010 to September 2016, 35 patients with 36 diabetic foot complicated with Wagner grade 2 or more foot ulcers were treated with saphenous neurocutaneous vascular flaps.  Results  The flaps had good blood supply, good wound healing and no necrosis. All patients were followed up for 3~12 months. The flaps were of good texture, good survival, tolerance to pressure, and most of the patients could recover the protective sensation. The foot could walk, and the function recovered satisfactorily. Conclusion The application of the saphenous neurocutaneous vascular flap for the diabetic foot ulcer has good clinical effecacy and can shorten the course of the disease. The operation, being simple and easy to popularize, can effectively improve the quality of life of patients and reduce the disability rate.

    Review in the clinical anatomy of the L5~S1 intervertebral foramen ligament
    ZHAO Qing-hao, LV Hai, DING Zi-hai
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  231-233.  doi:10.13418/j.issn.1001-165x.2018.02.022
    Abstract ( 436 )  
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    The progress of atlantoaxial joint distraction techniques in the management of  basilar invagination
    LAN Si-bin,DUAN Ming-yang,XU Jun-jie,ZHANG Qing-shun,YI Hong-lei,WU Zeng-hui
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  236-238.  doi:10.13418/j.issn.1001-165x.2018.02.024
    Abstract ( 543 )  
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    Morphologic malformation of the sternocleidomastoid muscle:one case report
    CUI Chun-ai, CUI Lin, LIU Huan-yu
    Chinese Journal Of Clinical Anatomy. 2018, 36(2):  239.  doi:10.13418/j.issn.1001-165x.2018.02.027
    Abstract ( 432 )  
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