Correlation between the degree of cerebral ischemia-reperfusion injury and the expression of LC3 and mTOR
LI Dan-dan,XIAO Xue-jin,QIN Shu-jian,BAO Cui-fen
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (2) : 182-186.
Correlation between the degree of cerebral ischemia-reperfusion injury and the expression of LC3 and mTOR
Objective To investigate the correlation between the degree of focal cerebral ischemia-reperfusion injury and the expression of LC3-Ⅱ and mTOR. Methods The models of focal cerebral ischemia-reperfusion in rats were established by occlusion of the middle cerebral artery. Neurological deficit score and TTC infarct volume were observed among groups. The expressions of LC3-Ⅱ and mTOR in different groups were detected by immunoblotting and immunofluorescence. Results Immunoblotting and immunofluorescence showed that LC3-Ⅱ had the highest expression in 24 hours after ischemia and reperfusion (IR 24 h), and mTOR was the lowest in IR 24h and IR 48 h. Correlation analysis showed that the infarcted volume of TTC was positively correlated with the expression level of LC3-II. Conclusion IR may activate the autophagy of the parietal neurons of the brain by increasing the expression level of LC3-II and decreasing the expression level of mTOR. The degree of cerebral ischemia-reperfusion injury may be related to the expression level of LC3 and mTOR.
Autophagy / Cerebral ischemia reperfusion / Autophagy microtubule associated protein light chain 3 antibody-Ⅱ / Rapamycin target protein antibody
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