Regulation of JNK/ERK signal pathway on Activin A mediated the recombination of F-actin of fibroblasts
CHEN Xiao-feng, ZHANG Min, WU Wei, ZHANG Lin
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (2) : 192-196.
Regulation of JNK/ERK signal pathway on Activin A mediated the recombination of F-actin of fibroblasts
Objective To explore whether Activin A regulate the filamentous actin of fibroblasts via MAPK signaling pathway. Methods Fibroblasts were Isolated and cultured from C57BL/6 rat skin which was born within 24 hours. The experiments were divided into PBS group, Activin A group, JNK specific inhibitor SP600125 group, ERK specific inhibitor SL327 group and p38 specific inhibitor SB202190 group. When cells were passaged for 3~5 generations, the total protein of cells was extracted, and the phosphorylation activity of JNK, ERK and p38 was detected by western blot. The changes of F-actin were observed by Phallotoxins staining. Results Activin A could induce the accumulation of F-actin in fibroblasts. After the use of JNK specific inhibitor SP600125, the F-actin aggregation of fibroblasts was completely inhibited. When the ERK specific inhibitor SL327 was used, the fibroblast F-actin aggregation was weakened, but not yet completely inhibited while P38 specific inhibitor SB202190 did not suppress the fibroblast F-actin aggregation. Conclusion Activin A can induce the F-actin recombination of fibroblasts via JNK and ERK signaling pathway.
Filamentous actin recombination / Activin A / MAPK signaling pathway / Fibroblasts
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