Screening of drug concentration of resveratrol in POI rats
LI Ning,LIU Li-li
Chinese Journal of Clinical Anatomy ›› 2018, Vol. 36 ›› Issue (2) : 206-210.
Screening of drug concentration of resveratrol in POI rats
Objective To observe the curative effect of different concentrations of resveratrol on rats with premature ovarian insufficiency (POI) and to determine the optimal drug concentration of resveratrol for treating premature ovarian insufficiency dysfunction in rats. Methods A total of 90 5-week-old SPF grade SD healthy female rats were randomly divided into a low-dose resveratrol group (18 rats in group a) and a middle-dose resveratrol group (18 rats in group b), a high dose resveratrol group (18 rats in group c), a model group (18 rats in group d) and a blank control group (18 rats in group e). Group a, group b, group c were administered with a daily dose of 20 mg/kg, 50 mg/kg, 80 mg/kg by gavage, respectively; the model control group was administered with 0.5% sodium carboxymethyl cellulose solution by gavage. ELISA was used to detect the level of serum sex hormone levels: anti-Mullerian hormone (AMH), luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E2) level and HE staining of rats to observe unit ovarian area of mature follicles, atresia follicles and corpus luteum, evaluation of resveratrol efficacy. Results Rats treated with different concentration of resveratrol had a significantly increased level of serum AMH and E2 compared to rats treated with 0.5% carboxymethyl cellulose solution (P<0.05), LH and FSH levels were lower (P<0.05).The serum level of AMH and E2 in POI rats treated with resveratrol at different concentration were lower (P<0.05) and LH and FSH were higher (P<0.05) than those of rats in the blank control. Compared with rats in group a, b, c and e, the number of granulosa cells, mature follicles and corpus luteum in ovaries was smaller in group d (P<0.05); In contrast, the number of atresia follicles was significantly increased in group d(P<0.05). Conclusion Intragastric treatment with different concentration of resveratrol of all have a certain effect on POI rats receiving, with 50 mg/kg showing the best result, and thus being the best drug concentration.
Premature ovarian insufficiency / Resveratrol / Optimal Drug concentration
[1] 中华医学会妇产科学分会绝经学组. 早发性卵巢功能不全的激素补充治疗专家共识[J]. 中华妇产科杂志, 2016, 51(12): 881-886.
[2] de Vet A, Laven JS, de Jong FH, et al. Antimüllerian hormone serum levels: a putative marker for ovarian aging[J]. Fertil Steril, 2002, 77(2): 357-362.
[3] La Marca A, Broekmans FJ, Volpe A, et al. AntiMullerian hormone (AMH): What do we still need to know[J]? Hum Reprod, 2009, 24(9): 22642275.
[4] Anderson RA. What does antiMüllerian hormone tell you about ovarian function[J]? Clin Endocrinol (Oxf), 2012, 77(5): 652655.
[5] Karkanaki A, Vosnakis C, Panidis D. The clinical significance of anti-Mullerian hormone evaluation in gynecological endocrinology[J]. Hormones ( Athens), 2011, 10(2): 95-103．
[6] Lie Fong S, Lugtenburg PJ, Schipper I, et al. AntiMüllerian hormone as a marker of ovarian function in women after chemotherapy and radiotherapy for haematological malignancies[J]. Hum Reprod, 2008, 23(3): 674678.
[7] Xia T, Fu Y, Gao H, et al. Recovery of ovary function impaired by chemotherapy using Chinese herbal medicine in a rat model[J]. Syst Biol Reprod Med, 2014, 60(5): 293303.
[8] Said RS, ElDemerdash E, Nada AS, et al. Resveratrol inhibits inflammatory signaling implicated in ionizing radiationinduced premature ovarian failure through antagonistic crosstalk between silencing information regulator 1 (SIRT1) and poly (ADPribose) polymerase 1 (PARP1) [J]. Biochem Pharmacol, 2016, 103: 140150.
[9] MolgaardHansen L, Skou AS, Juul A, et al. Pubertal development and fertility in survivors of childhood acute myeloid leukemia treated with chemotherapy only: a NOPHOAML study[J]. Pediatr Blood Cancer, 2013, 60(12): 19881995.
[10]Baur JA, Sinclair DA. The rapeutic potential of resveratrol:The in vivo evidence [J]. Nat Rev Drug Discov, 2006, 5(6): 493-506.
[11]曾又佳, 李顺民. 白藜芦醇拮抗雷公藤甲素卵巢毒性实验研究[J]. 新中医, 2014, 46(8): 172-174.
[12]Kong XX, Fu YC, Xu JJ, et al. Resveratrol, an effective regulator of ovarian development and oocyte apoptosis [J]. J Endocrinol Invest, 2011, 34 (11): 374-381.
[13]郝娟, 王春莲, 王培嵩, 等. 雷公藤多甙片致卵巢早衰大鼠动物模型的研究[J]. 实用妇产科杂志, 2012, 27(12): 1866-1870.
[14]黄辉屠, 润琪, 刘芳丽, 等. 白藜芦醇对慢性铅暴露小鼠脑组织Nrf2 信号通路的影响[J]. 卫生研究, 2015, 44(6): 954-958.
[15]Fu Y, Zhao Z, Wu Y, et al. Therapeutic mechanisms of Tongmai Dasheng Tablet on tripterygium glycosides induced rat model for premature ovarian failure[J]. Journal of ethnopharmacol, 2012, 139(1): 26-33.
[16] Özcan P, F?ç?c?o?lu C, Y?ld?r?m ÖK, et al. Protective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague-Dawley rats [J]. Reprod Biomed Online, 2015, 31(3): 404-410.
[17]刘长江, 毕文超, 张晨, 等. 白藜芦醇对大鼠脊髓损伤后炎症反应的影响[J]. 西安交通大学学报（医学版）, 2013, 34(6): 779-784.
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