Abstract:

Objective　  To study the effects of breviscapine on proliferation and apoptosis of scar-derived fibroblasts, for exploring new drugs to treat human pathological scar.　Methods　Fibroblasts were cultured as an experimental model and the effects of breviscapine were studied．Cell proliferation was determined by MTT assay. Apoptosis of cultured fibroblasts induced by breviscapine at the IC50 was detected by Annexin V/PI staining and flow-cytometry.　Results　In vitro，the proliferation of fibroblasts was significantly inhibited by breviscapine in the manner of dose-dependent(F=3.309，P<0.01). Compared with the control group, proliferation of fibroblasts was significantly inhibited in the concentration at 80,100,150 μmol/ml (P=0.015,0.006,0.000). Apoptosis rate of fibroblasts induced by breviscapine at the IC50 was significantly increased.　Conclusions　Breviscapine can significantly inhibit the proliferation and induce the apoptosis of cultured scar fibroblasts．It may be developed as a new drug for the treatment of pathological scar.

Key words:  Breviscapine, Fibroblasts, Apoptosis, Pathological scar