Rhizome panacis majoris total saponin inhibits human cytomegalovirus infection of human embryonic lung fibroblasts by up-regulating miR-216a

doi:10.13418/j.issn.1001-165x.2022.6.11

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Chinese Journal of Clinical Anatomy ›› 2022, Vol. 40 ›› Issue (6) : 689-695. DOI: 10.13418/j.issn.1001-165x.2022.6.11

Rhizome panacis majoris total saponin inhibits human cytomegalovirus infection of human embryonic lung fibroblasts by up-regulating miR-216a

Gao Shan¹, Pang Jie¹, Li Zhanjie², Li Chenxi³, Shang Qingyi⁴

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Abstract

Objective To explore the effect of rhizome panacis majoris total saponin on the inflammatory response and apoptosis of human embryonic fibroblast (MRC-5) infected by human cytomegalovirus (HCMV) and its regulatory effect on miR-216a. Methods MRC-5 cells were infected with HCMV to establish a virus infection model. The cells were treated with panacis majoris rhizome total saponin at different concentrations (100, 200, 400 μg/mL). The miR-NC and miR-216a mimics were transfected into HCMV-infected MRC-5 cells. After transfecting anti-miR-NC and anti-miR-216a into HCMV-infected MRC-5 cells, rhizome panacis majoris total saponin was added to the cells. ELISA was used to detect the levels of TNF-α and IL-6. Flow cytometry was used to detect the apoptosis rate. qRT-PCR method was used to detect the expression of miR-216a. Results Rhizome panacis majoris total saponin could reduce the apoptosis rate and the levels of TNF-α and IL-6 in MRC-5 cells infected with HCMV (P<0.05), while increase the expression level of miR-216a (P<0.05). Transfection of miR-216a mimics could reduce the rate of apoptosis and the levels of TNF-α and IL-6 (P<0.05). Transfection of anti-miR-216a could reduce the effect of rhizome panacis majoris total saponin on the inflammation and apoptosis of MRC-5 cells infected with HCMV. Conclusions Rhizome panacis majoris total saponin can inhibit the inflammatory response and apoptosis by up-regulating the expression of miR-216a, thereby reducing the damage of MRC-5 cells infected by HCMV.

Key words

Rhizome panacis majoris total saponin; / miR-216a; / Human cytomegalovirus; / Human embryonic fibroblast; / / Apoptosis; / / Inflammation

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Gao Shan, Pang Jie, Li Zhanjie, Li Chenxi, Shang Qingyi. Rhizome panacis majoris total saponin inhibits human cytomegalovirus infection of human embryonic lung fibroblasts by up-regulating miR-216a[J]. Chinese Journal of Clinical Anatomy. 2022, 40(6): 689-695 https://doi.org/10.13418/j.issn.1001-165x.2022.6.11

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