Objective To investigate the role of saikosaponin A (SA) in reducing hippocampal neuron damage in ischemia reperfusion (I/R) rats by up-regulating SIRT1 levels. Methods Rats were randomly divided into 6 groups: a sham operation group (Sham), a model (I/R) group, an I/R + SA 1mg/ kg group, an I/R + SA 5mg/kg group, an I/R + SA 10mg/kg group and an I/R + NMDP 1mg/kg group, 9 rats in each group. The cerebral ischemia-reperfusion model was constructed by the bilateral common carotid artery (CCA) with arterial clip clamping method, and it was administered intragastically for 7 days. The number of mistakes made by the jumping test and the number of new maze tests in the Y-maze test were recorded in each group of rats. Pathological damage of brain tissue was observed by HE staining. The 2,3,5-triphenyltetrazolium chloride method was used to calculate the cerebral infarction rate, brain tissue water content and brain index of each group of rats. Neuronal apoptosis was detected by Nissl body staining. The expression of Caspase3, Caspase9, Bax / Bcl-2 and SIRT1 was detected by Western blotting. The contents of superoxide dismutase (SOD), malondialdehyde (MDA), and lactate dehydrogenase (LDH) were detected by the kit. The expression of SIRT1 was detected by RT-PCR. Results Saikosaponin A reduced the number of mistakes in the platform-jumping experiment in rats, increased the number of new arms entering, reduced cerebral infarction rate, brain tissue water content, and brain index, and reduced Bax / Bcl-2, the ratio of Cleaved caspase3 and caspase3, Cleaved caspase9 and caspase9, decreased the content of MDA and LDH, increased the activity of SOD, and increased the expression level of SIRT1 (P<0.05). Conclusions Saikosaponin A alleviates hippocampal neuronal injury and oxidative stress in ischemia-reperfusion rats, which is related to the up-regulation of SIRT1.
Key words
Saikosaponin A /
SIRT1 /
Cerebral ischemia reperfusion /
Apoptosis /
Oxidative stress
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