Effects of LncRNA PVT1 on the growth and migration of cervical cancer HeLa cells via targeting miR-190

doi:10.13418/j.issn.1001-165x.2020.04.010

Chinese Journal of Clinical Anatomy ›› 2020, Vol. 38 ›› Issue (4) : 414-420. DOI: 10.13418/j.issn.1001-165x.2020.04.010

Effects of LncRNA PVT1 on the growth and migration of cervical cancer HeLa cells via targeting miR-190

DENG Yong-hong¹, ZHANG Li², WANG Jing¹, YAN Ai-hua¹

Author information +

History +

Abstract

Objective　To investigate the effects and mechanism of lncRNA PVT1 on the growth and migration of cervical cancer Hela cell via targeting miR-190.　Methods　The expression levels of lncRNA PVT1 and miR-190 in normal cervical cells and different cervical cancer cells were identified by qRT-PCR. The targeting relationship between lncRNA PVT1 and miR-190 was predicted by bioinformatics software. Cell proliferation was detected by MTT, cell growth was detected by clone formation assays, apoptosis was identified by Hoechst staining and flow cytometry, cell invasion was detected by Transwell, cell migration was detected by a scratch test; proliferation, apoptosis and epithelial-mesenchymal transition (EMT)-related molecular expression were verified by Western Blot. Cervical cancer HeLa cell was inoculated with subcutaneous xenografts of the nude mice after treated with sh-PVT1, and the related molecules expression of proliferation and apoptosis- in tumor tissues and tumor volume were detected.　Results　Compared with the normal cervical cells, lncRNA PVT1 was highly expressed in various cervical cancer cells. After interfering with PVT1, the expression level of miR-190 significantly up-regulated. LncRNA PVT1 and miR-190 had strong associativity, which was verified by bioinformatics and luciferase reporter system. Sh-PVT1 could effectively inhibit the proliferation, invasion and migration of cervical cancer cells and promote apoptosis (P<0.01). LncRNA PVT1 could effectively inhibit the related molecules expression of EMT- and reduce the transformation of cervical cancer cells into EMT. The above phenomenon was effectively reversed after the miR-190 inhibitor (P<0.01). In vivo experiments confirmed that PVT1 knockdown could effectively reduce tumor cell proliferation and promote cell apoptosis (P<0.01).　Conclusions　LncRNA PVT1 is highly expressed in cervical cancer. PVT1 knockdown can effectively relieve the inhibitory effect of PVT1 on miR-190, thereby reducing proliferation, invasion and migration of cervical cancer cells and promoting apoptosis.

Key words

LncRNA PVT1 / 　miR-190 / 　Cervical cancer / 　EMT / 　Growth / 　Apoptosis

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

DENG Yong-hong, ZHANG Li, WANG Jing, YAN Ai-hua. Effects of LncRNA PVT1 on the growth and migration of cervical cancer HeLa cells via targeting miR-190[J]. Chinese Journal of Clinical Anatomy. 2020, 38(4): 414-420 https://doi.org/10.13418/j.issn.1001-165x.2020.04.010

References

[1] 陈禹宏, 孟莹, 胡丽娜. 下调MARCH8基因的表达对宫颈癌Siha细胞侵袭、迁移与增殖的影响[J]. 中国免疫学杂志, 2018, 34(7): 13-17.
[2] Shi JF, Canfell K, Lew JB, et al. The burden of cervical cancer in china: synthesis of the evidence[J]. Int J Cancer, 2012, 130(3): 641-652.
[3] Sankaranarayanan R, Swaminathan R, Brenner H, et al. Cancer survival in africa, asia, and central america: a population-based study[J]. Lancet Oncol, 2010, 11(2): 165-173.
[4] Mattick JS, Makunin IV. Non-coding rna[J]. Hum Mol Genet, 2006, 15(1): R17-29.
[5] Chen YT, Bali D, Sullivan J. Prenatal diagnosis in glycogen storage diseases[J]. Prenat Diagn, 2002, 22(5): 357-359.
[6] Fatica A, Bozzoni I. Long non-coding rnas: New players in cell differentiation and development[J]. Nat Rev Genet, 2014, 15(1): 7-21.
[7] Heward JA, Lindsay MA. Long non-coding rnas in the regulation of the immune response[J]. Trends Immunol, 2014, 35(9): 408-419.
[8] Rossi MN, Antonangeli F. Lncrnas: new players in apoptosis control[J]. Int J Cell Biol, 2014, 5740(2014): 473857.
[9] De Coninck B, Carron D, Tavormina P, et al. Mining the genome of arabidopsis thaliana as a basis for the identification of novel bioactive peptides involved in oxidative stress tolerance [J]. J Exp Bot, 2013, 64(17): 5297-5307.
[10] 徐健, 江跃全, 蔡华荣, 等. miR-217调控lncRNA MALAT1影响食管鳞癌细胞的生物学行为[J]. 中国临床解剖学杂志, 2018, 36(4): 408-413.
[11] Yin J, Wen J, Hang D, et al. Expression quantitative trait loci for card8 contributes to risk of two infection-related cancers--hepatocellular carcinoma and cervical cancer[J]. PloS one, 2015, 10(7): e0132352.
[12] Wang XL, Wang GC, Zhang LJ, et al. Lncrna pvt1 promotes the growth of hpv positive and negative cervical squamous cell carcinoma by inhibiting tgf-beta1[J]. Cancer Cell Int, 2018, 18(1): 70.
[13]Shang CL, Wang W, Liao YD, et al. Lnmicc promotes nodal metastasis of cervical cancer by reprogramming fatty acid metabolism[J]. Cancer Res, 2018, 78(4): 877-890.
[14]Xue WS, Chen JJ, Liu XB, et al. PVT1 regulates the malignant behaviors of human glioma cells by targeting miR-190a-5p and miR-488-3p[J]. Biochim Biophys Acta Mol Basis Dis, 2018,1864(5): 173-1794.
[15]Chen W, Zheng R, Baade PD, et al. Cancer statistics in china, 2015[J]. CA Cancer J Clin 2016, 66(2): 115-132.
[16]Park S, Eom K, Kim J, et al. Mir-9, mir-21, and mir-155 as potential biomarkers for hpv positive and negative cervical cancer[J]. BMC cancer, 2017, 17(1): 658.
[17]Rui XH, Xu Y, Huang YQ, et al. Lncrna dlg1-as1 promotes cell proliferation by competitively binding with mir-107 and up-regulating zhx1 expression in cervical cancer[J]. Cell Physiol Biochem, 2018, 49(5): 1792-1803.
[18] Zhu H, Zheng T, Yu J, et al. Lncrna xist accelerates cervical cancer progression via upregulating fus through competitively binding with mir-200a[J]. Biomed Pharmacother, 2018, 105(1): 789-797.
[19] Cheng RJ, Li N, Yang SY, et al. Long non-coding rna zeb1-as1 promotes cell invasion and epithelial to mesenchymal transition through inducing zeb1 expression in cervical cancer[J]. Onco Targets Ther, 2018, 11(8): 7245-7253.
[20] Ma SY, Deng XH, Yang Y, et al. The lncrna linc00675 regulates cell proliferation, migration, and invasion by affecting wnt/beta-catenin signaling in cervical cancer[J]. Biomed Pharmacother, 2018, 108(2): 1686-1693.
[21] Liang HL, Zhang CY, Guan HY. Lncrna dancr promotes cervical cancer progression by upregulating rock1 via sponging mir-335-5p[J]. J Cell Physiol, 2019, 234(5): 7266-7278.
[22] Gao YL, Zhao ZS, Zhang MY, et al. Long noncoding rna pvt1 facilitates cervical cancer progression via negative regulating of mir-424[J]. Oncol Res, 2017, 25(8): 1391-1398.
[23] Shen CJ, Cheng YM, Wang CL. Lncrna pvt1 epigenetically silences mir-195 and modulates emt and chemoresistance in cervical cancer cells[J]. J Drug Target, 2017, 25(7): 637-644.
[24] Jia WZ, Yu T, An Q, et al. Microrna-190 regulates foxp2 genes in human gastric cancer[J]. Onco Targets Ther, 2016, 9(2): 3643-3651.