The regulatory effects of miR-143-3p on proliferation, apoptosis and invasion on human colon cancer cell via targeting MAPK1

doi:10.13418/j.issn.1001-165x.2019.03.011

Chinese Journal of Clinical Anatomy ›› 2019, Vol. 37 ›› Issue (3) : 292-298. DOI: 10.13418/j.issn.1001-165x.2019.03.011

ZHANG Zhi-qian¹, ZHANG Guang-xin², PENG Xiao-dong²

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Abstract

Objective　To investigate the relationship between miR-143-3p targeting MAPK1 and its effect on proliferation, apoptosis and invasion of human colon cancer cells.　Methods　qRT-PCR was used to detect the effect of transfection and the expression level of MAPK1 mRNA. Double luciferase reporter assay was used to detect the targeting relationship between microRNAs-143-3p and MAPK1. The expression level of MAPK1 protein was detected by Western blot. The proliferation multiple was detected by CCK-8. The proliferation was detected by Hoechst staining. The cell invasion was detected by in vitro invasion test. The expression of Ki67, VEGF，MMP-2 and claspase-3 was detected by Western blot.　Results　miR-143-3p mimic inhibited the expression of MAPK1 mRNA and protein in SW620 cells. miR-143-3p mimic and MAPK1 wild-type reporter plasmid decreased the activity of luciferase significantly. After miR-143-3p mimic transfection, the proliferation and invasion of SW620 cells were significantly decreased, the number of apoptotic cells was significantly increased, the expression of Ki67, VEGF and MMP-2 was significantly decreased, and the expression of cl-caspase-3 was significantly increased. The miR-143-3p mimic alleviated the induction effect of high expression of MAPK1 on proliferation and invasion of SW620 cells and inhibition effect on cell apoptosis.　Conclusion　miR-143-3p can inhibit proliferation and invasion of human colon cancer cells and induce apoptosis through possible targeting inhibition of MAPK1.

Key words

Colon cancer / 　Micro RNA / 　MAPK1 / 　Proliferation / 　Apoptosis / 　Invasion

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ZHANG Zhi-qian, ZHANG Guang-xin, PENG Xiao-dong. The regulatory effects of miR-143-3p on proliferation, apoptosis and invasion on human colon cancer cell via targeting MAPK1[J]. Chinese Journal of Clinical Anatomy. 2019, 37(3): 292-298 https://doi.org/10.13418/j.issn.1001-165x.2019.03.011

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