莫诺苷调控miR-483-5p表达对高糖诱导的人视网膜血管内皮细胞氧化应激和凋亡影响

doi:10.13418/j.issn.1001-165x.2023.4.11

摘要/Abstract

摘要： 目的探讨莫诺苷（Morroniside，Mor）对高糖诱导的人视网膜血管内皮细胞（human retinal microvascular endothelial cells，HRECs）氧化应激、凋亡的影响及其分子机制。方法将HRECs分为对照组（Con）、高糖组（HG）、HG+Mor-低剂量组（L）、HG+Mor-中剂量组（M）、HG+Mor-高剂量组（H）、HG+anti-miR-NC组、HG+anti-miR-483-5p组、HG+Mor+miR-NC组、HG+Mor+miR-483-5p组。流式细胞仪检测HRECs凋亡率及ROS水平，酶联免疫吸附检测MDA水平和SOD活性。实时定量PCR检测miR-483-5p表达。结果与Con组比较，HG组HRECs凋亡率、ROS和MDA水平升高（P<0.05），SOD活性降低（P<0.05），miR-483-5p表达升高（P<0.05）。与HG组比较，HG+Mor-L组、HG+Mor-M组、HG+Mor-H组HRECs凋亡率、ROS和MDA水平降低（P<0.05），SOD活性升高（P<0.05），miR-483-5p表达降低（P<0.05）。与HG+anti-miR-NC组比较，HG+anti-miR-483-5p组HRECs凋亡率、ROS和MDA水平降低（P<0.05），SOD活性升高（P<0.05）。与HG+Mor+miR-NC组比较，HG+Mor +miR-483-5p组HRECs凋亡率、ROS和MDA水平升高（P<0.05），SOD活性降低（P<0.05）。结论莫诺苷通过下调miR-483-5p表达可抑制高糖诱导的HRECs氧化应激和凋亡。

关键词: 莫诺苷； , , miR-483-5p； , , 高糖； , , 视网膜； , , 血管内皮细胞； , , 氧化应激； , , 凋亡

Abstract: Objective To investigate the effect and molecular mechanism of Morroniside (Mor) on high glucose-induced human retinal microvascular endothelial cells (HRECs) oxidative stress and apoptosis. Methods HRECs were divided into a control (Con) group, a high glucose (HG) group, a HG+Mor-low dose (L) group, a HG+Mor-medium dose (M) group, a HG+Mor-high dose (H) Group, a HG+anti-miR-NC group, a HG+anti-miR-483-5p group, a HG+Mor+miR-NC group, a HG+Mor+miR-483-5p group. The apoptosis rate and ROS level of HRECs were detected by using flow cytometry. MDA level and SOD activity were assessed by using enzyme-linked immunosorbent assay. miR-483-5p expression was detected by using Real-time quantitative PCR. Results Compared with the Con group, the apoptosis rate, ROS and MDA levels of HRECs in the HG group were significantly increased (P<0.05), SOD activity was significantly decreased (P<0.05), and miR-483-5p expression was significantly increased (P<0.05). Compared with the HG group, the apoptosis rate, ROS and MDA levels of HRECs in the HG+Mor-L group, HG+Mor-M group, and HG+Mor-H group were significantly reduced (P<0.05), and SOD activity was significantly increased (P<0.05), miR-483-5p expression was significantly reduced (P<0.05). Compared with the HG+anti-miR-NC group, the apoptosis rate, ROS and MDA levels of HRECs in the HG+anti-miR-483-5p group were significantly reduced (P<0.05), and SOD activity was significantly increased (P<0.05). Compared with the HG+Mor+miR-NC group, the apoptosis rate, ROS and MDA levels of HRECs in the HG+Mor+miR-483-5p group were significantly increased (P<0.05), and SOD activity was significantly decreased (P<0.05). Conclusions Morroniside inhibits high glucose-induced HRECs oxidative stress and apoptosis by down-regulating miR-483-5p expression.

Key words: Morroniside; , , miR-483-5p; , , High glucose; , , Retina; , , Vascular endothelial cells, Oxidative stress; , , Apoptosis

中图分类号:

R774.1

陈平, 魏雪梅, 谈丽丽. 莫诺苷调控miR-483-5p表达对高糖诱导的人视网膜血管内皮细胞氧化应激和凋亡影响[J]. 中国临床解剖学杂志, 2023, 41(4): 434-439.

Chen Ping, Wei Xuemei, Tan Lili. Effect of morroniside on high glucose-induced human retinal vascular endothelial cells oxidative stress and apoptosis by regulating miR-483-5p expression[J]. Chinese Journal of Clinical Anatomy, 2023, 41(4): 434-439.

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