关键词: mir-325-3p； ,  , RIPK3/TFEB； ,  , 脑出血； ,  , 神经元损伤

Abstract: Objective   To investigate the effect of mir-325-3p on neuronal injury in rats with intracerebral hemorrhage by regulating RIPK3/TFEB pathway.    Methods    SD male rats were randomly divided into a sham operation group, an intracerebral hemorrhage group, an agomir NC group, an agomir-325-3p group, a GSK872 (RIPK3 inhibitor) group, and an agomir-325-3p+GSK872 group, with 18 rats in each group. The motor function, limb coordination ability and modified neurological deficit score (MNSs) of rats were evaluated. The brain water content, pathological changes of brain tissue around hematoma, neuronal apoptosis, autophagy of rats, CO localization of LC3 and NeuN, RIPK3/TFEB signal pathway in hippocampus and the expression of autophagy related proteins were measured.    Results    Compared with the sham operation group, the neurons in CA1 area of intracerebral hemorrhage group were swollen and loosely arranged, with a large number of lysosomes and autophagy bodies, the success rate of forelimb placement, the percentage of left turn and TFEB decreased (P<0.05), and the MNSs score, brain water content, the rate of neuronal apoptosis, RIPK 3, Beclin-1, LC3-II / LC3 - Ⅰ and LC3 / NeuN strong positive numbers increased (P<0.05). Agomir-325-3p and GSK872 can reduce the damage of hippocampal neurons and enhance autophagy.    Conclusions    Mir-325-3p overexpression may promote autophagy and reduce neuronal damage in rats with intracerebral hemorrhage by regulating RIPK3 / TFEB.

Key words: mir-325-3p; ,  , RIPK3/TFEB; ,  ,  cerebral hemorrhage; ,  ,  neuronal damage