Efficacy of umbilical cord extract on hair and hair follicles

Wu Shenhua, Wang Xueer, Fan Xiaoyu, Zhang Min, Zhang Lin

doi:10.13418/j.issn.1001-165x.2025.3.11

Chinese Journal of Clinical Anatomy >

2025 , Vol. 43 >Issue 3: 313 - 322

DOI: https://doi.org/10.13418/j.issn.1001-165x.2025.3.11

Efficacy of umbilical cord extract on hair and hair follicles

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1.Department of Histology and Embryology, GDMPA Key Laboratory of Key Technologies for Cosmetics Safety and Efficacy Evaluation, NMPA Key Laboratory for Safety Evaluation of Cosmetics, Key Laboratory of Construction and Detection in Tissue Engineering of Guangdong Province, Guangdong Provincial Key Laboratory of Functional Proteomics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, Guangdong Province, China; 2. Ultra Biotech (Guangzhou) Ltd. co., Guangzhou 510535, Guangdong Province, China.

Received date: 2024-12-26

Online published: 2025-06-24

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Abstract

Objective To explore and develop animal-derived functional cosmetic raw materials, pig umbilical cord extracts were tested for protein concentration, subjected to metabolomics and proteomics analysis, and their efficacy on hair follicle cells, animal hair follicle cycles, and androgenetic alopecia (AGA) models was studied. Methods The protein concentration and metabolomic analysis of porcine umbilical cord extract were carried out, and the evaluation of the efficacy of the hair follicle cycle and the efficacy of mouse hair follicle cycle was completed by establishing a mouse hair follicle cycle model and treating mice with different concentrations of porcine umbilical cord extract and tracking the skin color, hair follicle changes and hair diameter of mice, and analyzing the histological morphology of mouse hair follicles. The histomorphology of hair follicles in mice was analyzed, the levels of androgen receptor and Dickkopf-related protein 1 (DKK1) were detected, and the changes of skin blood flow were tracked to complete the efficacy evaluation of the androgenetic alopecia model. Results (1) The protein concentration of pig umbilical cord extract extracted by patented extraction technology was (7.45±1.23) mg/ml; (2) The number of proteins and peptides of pig umbilical cord extract, the number of available proteins: (187±12), the number of peptides: (696±44) (n=3); (3) The hair diameter of the back hair follicles of mice increased after treatment with porcine umbilical cord extract; (4) The number of hair follicles entering the growth phase increased significantly after the porcine umbilical cord extract was applied to the dorsal skin of mice (15-90 μg/mL), and the size and number of hair follicles gradually increased；(5) After the umbilical cord extract was applied, the hair coverage of the back skin increased, the hair bulb of hair follicles increased, the expression of AR and DKK1 decreased, and the blood flow increased. Conclusions (1) Porcine umbilical cord extract contains abundant and diverse small molecule metabolites, a variety of proteins, polypeptides and other substances; (2) Porcine umbilical cord extract can promote the thickening of hair follicles in mice, and accelerate the transition from telogen phase to growth phase; (3) Umbilical cord extract can improve the average blood perfusion in the area of hair loss and inhibit the miniaturization of hair follicles caused by androgenetic alopecia by inhibiting the expression of AR and DKK1.

Key words： Umbilical cord extract; ; ; Hair follicle cycle; ; ; Androgenetic alopecia（AGA）; ; ; Wnt/β-catenin

Cite this article

Wu Shenhua, Wang Xueer, Fan Xiaoyu, Zhang Min, Zhang Lin . Efficacy of umbilical cord extract on hair and hair follicles[J]. Chinese Journal of Clinical Anatomy, 2025 , 43(3) : 313 -322 . DOI: 10.13418/j.issn.1001-165x.2025.3.11

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