收稿日期: 2015-05-25
网络出版日期: 2016-01-28
基金资助
河北省卫生厅自然科学基金（20150016）
Analysis of the relationship between the changes of 5-HT2A receptor in spinal cord and the mouse tail spasm after spinal cord injury
Received date: 2015-05-25
Online published: 2016-01-28
目的 通过损伤大鼠脊髓腰1节段来探讨损伤下段5-HT2A受体免疫反应变化与鼠尾痉挛状态的关系，为治疗患者脊髓损伤后肢体痉挛提供理论依据。 方法 60只雄性Wistar大鼠随机分为空白组、打击组和横断组（n=20），用免疫组化法研究5-HT2A受体免疫反应的变化，并分析与鼠尾痉挛状态的关系。所得数据统计学处理。 结果 (1)打击组和横断组5-HT2A受体免疫反应的密度比空白组增高，横断组增高更显著，差异有统计学意义。(2)脊髓损伤后鼠尾痉挛状态与5-HT2A受体的密度和脊髓损伤的程度呈正相关。 结论 脊髓损伤后鼠尾痉挛状态与5-HT2A受体密度的变化呈正相关，应用5-HT2A受体阻滞剂治疗患者的肢体痉挛提供了理论依据。
王永为 , 李海艳 , 高亚贤 , 郭森 , 谢红林 . 脊髓损伤后5-HT2A受体的变化与鼠尾痉挛关系的分析[J]. 中国临床解剖学杂志, 2016 , 34(1) : 83 -86 . DOI: 10.13418/j.issn.1001-165x.2016.01.021
Objective To study the relationship between the immunoreactive changes of 5-HT2A receptor and tail Spasticity, within spinal L1 segment after SCI, and to provide a theoretical basis for The treatment of spinal cord injury. Methods Sixty adult males Wistar rats were randomly separated into three groups: control group, attacked group and transversed group. To study 5-HT2AR immunoreactivity and hyperexcitability of tail of rats. Results (1) Compared the attacked and transversed groups with the control group, 5-HT2AR-IR density significantly increased in both attacked and transversed groups compared with that in the control group. (2) Spasticity and reflective hyperfunction symptom after SCI were positively correlated with 5-HT2AR-IR density and extent of SCI. Conclusion Spasticity and reflective hyperfunction symptom after SCI are positively correlated wtih 5-HT2AR-IR density and the extent of SCI, which provides the theoretical basis for using 5-HT2A receptor blockers in patients with limb spasm.
Key words： Spinal cord injury; 5-HT2A receptor; Spasticity
[1] Khan T, Havey RM, Sayers ST, et al. Animal model of spinal cord contusion injuries[J]. Lab Anim Sci, 1999, 49(2): 161-172.
[2] Kong XY, Wienecke J, Hultborn H, et al. Robust upregulation of serotonin 2A receptors after chronic spinal transection of rats: an immunohistochemical study[J]. Brain Research, 2010, 13(20):60-68.
[3] Nielsen JB, Crone C, Hultborn H. The spinal pathophysiology of spasticity-from a basic science point of view[J].Acta Physiologica, 2007,189(2):171-180.
[4] Kong XY, Wienecke J, Chen M, et al. The time course of serotonin 2A receptor expression after spinal transection of rats: an immuno- histochemical study[J].Neuroscience, 2011,177:114-126.
[5] 郭世绂, 胥少汀.脊髓损伤基础与临床[M].北京:人民卫生出版社, 2003:181-293.
[6] Schmidt BJ, Jordan LM. The role of serotonin in reflex modulation and locomotor rhythm production in the mammalian spinal cord[J]. Brain Res, 2000, 53(5): 689-710.
[7] Fonseca MI, Ni YG, Dunning DD, et al. Distribution of serotonin 2A, 2C and 3 receptor mRNA in spinal cord and medulla oblongata[J]. Brain Res Mol Brain Res, 2001, 89(2): 11-19.
[8] Ung RV, Landry ES, Rouleau P, et al. Role of spinal 5-HT2 receptor subtypes in quipazine-induced hindlimb movements after a low-thoracic spinal cord transection[J]. Eur Neuroscienc，2008, 28(11): 2231-2242.
[9] Landry ES, Guertin PA. Differential effects of 5-HT1 and 5-HT2 receptor agonists on hindlimb movements in the paraplgic mice[J]. Prog Neuropsychopharmacol Biol Psychiatry, 2004, 2(8):1053-1060.
[10] Bennett DJ, Sanelli L, Cooke CL, et al. Spastic long-lasting reflexes in the awake at after sacral Spinal cord injury [J]. J Neurophysiol, 2004, 91(5):2247-2258.
[11] Bedard P, Barbeau H, Barbeau G, et al. Progressive increase of motor activity induced by 5-HTP in the rat below a complete section of the spinal cord[J]. Brain Res, 1979,169(2):393-397.
[12] Hulton H, Brownstone RB, Toth TI, et al. Key mechanisms for setting the input-output gain across the motoneuron pool[J]. Prog Brain Res，2004,143(1):77-95.
[13] 大鼠脊髓切断术后神经纤维残留量与运动功能恢复的关系[J].解剖学报，2010, 32(2):127-131.
/
| 〈 |
|
〉 |
京ICP备08002354号-3
