脊髓损伤后5-HT2A受体的变化与鼠尾痉挛关系的分析
Analysis of the relationship between the changes of 5-HT2A receptor in spinal cord and the mouse tail spasm after spinal cord injury
目的 通过损伤大鼠脊髓腰1节段来探讨损伤下段5-HT2A受体免疫反应变化与鼠尾痉挛状态的关系，为治疗患者脊髓损伤后肢体痉挛提供理论依据。 方法 60只雄性Wistar大鼠随机分为空白组、打击组和横断组（n=20），用免疫组化法研究5-HT2A受体免疫反应的变化，并分析与鼠尾痉挛状态的关系。所得数据统计学处理。 结果 (1)打击组和横断组5-HT2A受体免疫反应的密度比空白组增高，横断组增高更显著，差异有统计学意义。(2)脊髓损伤后鼠尾痉挛状态与5-HT2A受体的密度和脊髓损伤的程度呈正相关。 结论 脊髓损伤后鼠尾痉挛状态与5-HT2A受体密度的变化呈正相关，应用5-HT2A受体阻滞剂治疗患者的肢体痉挛提供了理论依据。
Objective To study the relationship between the immunoreactive changes of 5-HT2A receptor and tail Spasticity, within spinal L1 segment after SCI, and to provide a theoretical basis for The treatment of spinal cord injury. Methods Sixty adult males Wistar rats were randomly separated into three groups: control group, attacked group and transversed group. To study 5-HT2AR immunoreactivity and hyperexcitability of tail of rats. Results (1) Compared the attacked and transversed groups with the control group, 5-HT2AR-IR density significantly increased in both attacked and transversed groups compared with that in the control group. (2) Spasticity and reflective hyperfunction symptom after SCI were positively correlated with 5-HT2AR-IR density and extent of SCI. Conclusion Spasticity and reflective hyperfunction symptom after SCI are positively correlated wtih 5-HT2AR-IR density and the extent of SCI, which provides the theoretical basis for using 5-HT2A receptor blockers in patients with limb spasm.
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河北省卫生厅自然科学基金（20150016）
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