目的 使用自凝硫酸钡复合材料进行动静脉灌注铸型，以优化离体胎盘组织的计算机断层血管造影（CTA）影像质量。 方法 将60个正常、足月分娩的胎盘随机分为两组，分别使用传统造影剂和自凝硫酸钡复合材料灌注后进行CT扫描和3D重建。 结果 自凝硫酸钡复合材料显著提升了CTA血管充盈度和分支清晰度，铸型后的CTA影像质量优于传统方法。 结论 自凝硫酸钡复合材料在离体胎盘血管研究中表现出显著优势，有助于胎盘生理和病理研究，为相关疾病的诊治提供新的手段，提高临床诊断的准确性。
Objective To optimize the imaging of computed tomography angiography (CTA) of ex vivo placental tissue by using a self-curing barium sulfate composite material for arteriovenous casting. Methods Normal full-term placentas were selected and randomly divided into two groups. Each group underwent CT scanning and 3D reconstruction using either traditional contrast agents or the self-curing barium sulfate composite material. Results The self-curing barium sulfate composite significantly enhanced the vascular filling and branch clarity in the CTA images. Blinded visual scoring demonstrated that the quality of CTA images obtained with the casting method surpassed those obtained with traditional methods, validating its effectiveness in ex vivo placental vascular studies. Conclusions The self-curing barium sulfate composite material shows significant advantages in ex vivo placental vascular research, improving the accuracy of placental pathology studies and clinical diagnoses. It provides new methodologies for diagnosing and treating related diseases.
[1] Turco MY, Gardner L, Kay RG, et al. Trophoblast organoids as a model for maternal-fetal interactions during human placentation[J]. Nature, 2018, 564(7735): 263-267. DOI: 10.1038/s41586-018-0753-3.
[2] Hitzerd E, Reijnders IF, Mulders A, et al. Larger first-trimester placental volumetric parameters are associated with lower pressure and more flow-mediated vasodilation of the fetoplacental vasculature after delivery[J]. Front Physiol,2020,11:6. DOI: 10.3389/fphys. 2020. 00006.
[3] Moran CM, Thomson AJW. Preclinical ultrasound imaging-a review of techniques and imaging applications[J]. Front Phys, 2020, 8: 124. DOI: 10.3389/fphy.2020.00124.
[4] Melbourne A, Schabel MC, David AL, et al. Magnetic resonance imaging of placental Intralobule structure and function in a pre-clinical nonhuman primate model[J]. Biol Reprod, 2024, 110(6): 1065-1076. DOI: 10.1093/biolre/ioae035.
[5] Link D, Many A, Ben Sira L, et al. Placental vascular tree characterization based on ex-vivo MRI with a potential application for placental insufficiency assessment[J]. Placenta, 2020, 101: 252-260. DOI: 10.1016/j.placenta.2020.05.001.
[6] Lê MP, Pencolé L, Peytavin G, et al. Placental transfer of doravirine, a recent HIV-1 NNRTI in the ex vivo human cotyledon perfusion model[J]. J Antimicrob Chemother, 2021, 76(9): 2364-2367. DOI: 10.1093/jac/dkab202.
[7] Collet C, Chevalier B, Cequier A, et al. Diagnostic accuracy of coronary ct angiography for the evaluation of bioresorbable vascular scaffolds[J]. JACC Cardiovasc Imaging, 2018, 11(5): 722-732. DOI: 10.1016/j.jcmg.2017.04.013.
[8] Pearce SC, Coia HG, Karl JP, et al. Intestinal in vitro and ex vivo models to study host-microbiome interactions and acute stressors[J]. Front Physiol, 2018, 9: 1584. DOI: 10.3389/fphys.2018.01584.
[9] 张家栋, 李长征, 陈跃, 等. 改良自凝牙托材料在胎盘血管铸型中的应用[J]. 福建中医学院学报, 2003, 13(2): 33. DOI: 10.3969/j.issn.1004-5627.2003.02.017.
Zhang JD, Li CZ, Chen Y, et al. Improved Self-Curing Dental Baseplate Material in Placental Vascular Casting Application. Journal of Fujian College of TCM, 2003, 13(2): 33. DOI: 10.3969/j.issn.1004-5627.2003.02.017.
[10]Walter A, Paul-Gilloteaux P, Plochberger B, et al. Correlated multimodal imaging in life sciences: expanding the biomedical horizon[J]. Front Phys, 2020, 8: 47. DOI: 10.3389/fphy.2020.00047.
[11]Ahmed A, Hamza HM. Barium sulfate absorption and sensitivity[J]. Radiology, 1989, 172(1): 213-214. DOI: 10.1148/radiology.172.1.213-b.
[12]Xu Y, Huettig F, Schille C, et al. Peel bond strength between 3D printing tray materials and elastomeric impression/adhesive systems: a laboratory study[J]. Dent Mater, 2020, 36(7): e241-e254. DOI: 10.1016/j.dental.2020.04.015.
[13]Elbæk Madsen K, Mariager C, Duvald CS, et al. Ex vivo human placenta perfusion, metabolic and functional imaging for obstetric research-a feasibility study[J]. Tomography, 2019, 5(4): 333-338. DOI: 10.18383/j.tom.2019.00016.
京ICP备08002354号-3
