目的 研究马蹄内翻足畸形发生的分子机制。 方法 采用HE染色观察两组胎鼠足踝部组织结构差异，免疫组化染色、qRT-PCR和Western blot技术检测模型组和对照组大鼠足踝部组织缺氧诱导因子1（hypoxia inducible factor 1，HIF-1）、血管内皮生长因子（vascular endothelial growth factor，VEGF）及Notch-1表达情况。 结果 光镜下观察，与对照组相比，实验组足踝部组织结构相对紊乱，组织间隙增大，软组织松散，局部软组织聚集挛缩。免疫组化染色、qRT-PCR和Western blot结果显示，实验组足踝部组织HIF-1表达水平下调，Vegf、Notch-1的表达减少。 结论 HIF-VEGF-Notch信号通路可能与马蹄内翻足畸形的发生相关。
Objective To study the molecular mechanism of equinovarus deformity. Methods HE staining was used to observe the difference of foot and ankle tissue structure in model group and control group. The expressions of hypoxia inducible factor 1 (HIF-1), vascular endothelial growth factor (VEGF) and Notch-1 in the tissues of foot and ankle of rats in model group and control group were detected by immunohistochemical staining, Western Blot and qRT-PCR. Results Microscopic observation results showed that compared with the control group, the tissue structure of the foot and ankle in the experimental group was relatively disordered, with enlarged tissue space, loose soft tissue and aggregation contracture of local soft tissue. Immunohistochemical staining, Western Blot and qRT-PCR results showed that compared with the control group, the expression of HIF-1 down-regulated in ankle tissue of the experimental group, and the expression of VEGF and Notch-1 decreased. Conclusions HIF-VEGF-Notch signaling pathway may be related to the occurrence of varus foot deformity.
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