缺氧诱导因子1-血管内皮生长因子-Notch信号通路参与马蹄内翻足畸形的发生机制

doi:10.13418/j.issn.1001-165x.2022.1.12

中国临床解剖学杂志 ›› 2022, Vol. 40 ›› Issue (1): 62-66.doi: 10.13418/j.issn.1001-165x.2022.1.12

缺氧诱导因子1-血管内皮生长因子-Notch信号通路参与马蹄内翻足畸形的发生机制

张真翊1，陈曦2，王谦3*

1.中国医科大学，沈阳 110122； 2.沈阳医学院基础医学院，沈阳 110034；
3.中国医科大学生命科学学院医学基因组教研室，沈阳 110122

收稿日期:2021-02-10 出版日期:2022-01-25 发布日期:2022-01-19
通讯作者: 王谦，博士，副教授，E-mail：wangqian529@163.com
作者简介:张真翊（2000-），女，辽宁辽阳人，临床本科在读，E-mail：Zhangzhenyi2021@126.com
基金资助:
辽宁省大学生创新创业训练计划（S202010164003）

HIF-VEGF-Notch signaling pathway may be involved in the occurrence of equinovarus deformity

Zhang Zhenyi1, Chen Xi2，Wang Qian3*

1. China Medical University, Shenyang 110122, China; 2. Basic Medical School of Shenyang Medical College, Shenyang 110034, China; 3. Department of Medical Genomics, School of Life Sciences, China Medical University, Shenyang 110122, China

Received:2021-02-10 Online:2022-01-25 Published:2022-01-19

摘要/Abstract

摘要： 目的　研究马蹄内翻足畸形发生的分子机制。方法　采用HE染色观察两组胎鼠足踝部组织结构差异，免疫组化染色、qRT-PCR和Western blot技术检测模型组和对照组大鼠足踝部组织缺氧诱导因子1（hypoxia inducible factor 1，HIF-1）、血管内皮生长因子（vascular endothelial growth factor，VEGF）及Notch-1表达情况。结果　光镜下观察，与对照组相比，实验组足踝部组织结构相对紊乱，组织间隙增大，软组织松散，局部软组织聚集挛缩。免疫组化染色、qRT-PCR和Western blot结果显示，实验组足踝部组织HIF-1表达水平下调，Vegf、Notch-1的表达减少。结论　HIF-VEGF-Notch信号通路可能与马蹄内翻足畸形的发生相关。

关键词: 缺氧诱导因子1, 　血管内皮生长因子, 　Notch信号通路, 　马蹄内翻足模型

Abstract: Objective　To study the molecular mechanism of equinovarus deformity.　Methods　HE staining was used to observe the difference of foot and ankle tissue structure in model group and control group. The expressions of hypoxia inducible factor 1 (HIF-1), vascular endothelial growth factor (VEGF) and Notch-1 in the tissues of foot and ankle of rats in model group and control group were detected by immunohistochemical staining, Western Blot and qRT-PCR.　Results　Microscopic observation results showed that compared with the control group, the tissue structure of the foot and ankle in the experimental group was relatively disordered, with enlarged tissue space, loose soft tissue and aggregation contracture of local soft tissue. Immunohistochemical staining, Western Blot and qRT-PCR results showed that compared with the control group, the expression of HIF-1 down-regulated in ankle tissue of the experimental group, and the expression of VEGF and Notch-1 decreased.　Conclusions 　HIF-VEGF-Notch signaling pathway may be related to the occurrence of varus foot deformity.

Key words: Hypoxia inducible factor 1 (HIF1), 　Vascular endothelial growth factor (VEGF), 　Notch signaling pathway, 　Congenital talipes equinovarus

中图分类号:

Q291

张真翊, 陈曦, 王谦. 缺氧诱导因子1-血管内皮生长因子-Notch信号通路参与马蹄内翻足畸形的发生机制[J]. 中国临床解剖学杂志, 2022, 40(1): 62-66.

Zhang Zhenyi, Chen Xi, Wang Qian. HIF-VEGF-Notch signaling pathway may be involved in the occurrence of equinovarus deformity[J]. Chinese Journal of Clinical Anatomy, 2022, 40(1): 62-66.

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缺氧诱导因子1-血管内皮生长因子-Notch信号通路参与马蹄内翻足畸形的发生机制

HIF-VEGF-Notch signaling pathway may be involved in the occurrence of equinovarus deformity

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