介绍一种免疫组化标签的改进设计及应用

doi:10.13418/j.issn.1001-165x.2026.3.18

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中国临床解剖学杂志 ›› 2026, Vol. 44 ›› Issue (3) : 368-370. DOI: 10.13418/j.issn.1001-165x.2026.3.18

技术方法

介绍一种免疫组化标签的改进设计及应用

董杏梅¹，王瑞武²，李明显¹*

作者信息 +

The improved design and application of an immunohistochemical label

Dong Xingmei¹, Wang Ruiwu², Li Mingxian¹*

Author information +

文章历史 +

摘要

目的探讨如何改进免疫组化标签的设计，使其简单的提示信息能够在不同的工作环节均能成为有效的参考信息，从而简化工作流程，提高工作效率及工作质量。方法在免疫组化标签的不同位置上分别增加代表不同免疫组化仪器、不同组织芯片、不同病理医生的编码，在不同的工作环节参考相应的编码信息，比较对照组和实验组的时间消耗和准确率。结果技术人员能够快速准确地完成免疫组化切片的标签粘贴及切片分组工作。结论改进设计的免疫组化标签能够提高技术人员的工作效率和工作质量。

Abstract

Objective This study explores the redesign of immunohistochemical (IHC) labels to incorporate concise prompts that serve as effective reference information across multiple workflow stages, thereby streamlining processes and improving efficiency and quality. Methods Distinct coding systems were added to specific sections of IHC labels to represent different IHC instruments, tissue microarrays, and pathologists. Time consumption and accuracy were compared between control and experimental groups by referencing relevant codes at respective workflow stages. Results Technicians were able to complete label application and slide sorting for IHC slides with improved speed and accuracy. Conclusion Optimized IHC labels enhance technicians’ operational efficiency and quality of work.

导出引用

董杏梅, 王瑞武, 李明显. 介绍一种免疫组化标签的改进设计及应用[J]. 中国临床解剖学杂志. 2026, 44(3): 368-370 https://doi.org/10.13418/j.issn.1001-165x.2026.3.18

Dong Xingmei, Wang Ruiwu, Li Mingxian. The improved design and application of an immunohistochemical label[J]. Chinese Journal of Clinical Anatomy. 2026, 44(3): 368-370 https://doi.org/10.13418/j.issn.1001-165x.2026.3.18

中图分类号： R329.33

参考文献

[1] 杨月红, 袁静萍, 袁修学, 等. 自动免疫组化仪的使用体会[J]. 临床与实验病理学杂志,2011,27(3):333-334. DOI:10.13315/j.cnki.cjcep. 2011.03.004.
Yang YH, Yuan JP, Yuan XX, et al. Experience in using an automated immunohistochemistry instrument[J]. Journal of Clinical and Experimental Pathology, 2011, 27(3): 333-334. DOI:10.13315/j.cnki.cjcep.2011.03.004.
[2] 吴兴旗, 党啟华, 徐晶晶, 等. 全自动免疫组化染色仪比较不同抗原修复液对p53的染色结果[J]. 临床与实验病理学杂志, 2024, 40(6):663-664. DOI:10.13315/j.cnki.cjcep.2024.06.022.
Wu XQ, Dang QH, Xu JJ, et al. Comparison of staining results of p53 by automatic immunohistochemical stainer using different antigen retrieval solutions[J]. Journal of Clinical and Experimental Pathology, 2024, 40(6): 663-664. DOI:10.13315/j.cnki.cjcep.2024.06.022.
[3] 陈杰伟, 刘君, 卢佳斌, 等. Leica、Dako和Roche的免疫组化自动化染色平台应用体会[J]. 临床与实验病理学杂志, 2017, 33(4): 465-467.DOI:10.13315/j.cnki.cjcep.2017.04.031.
Chen JW, Liu J, Lu JB, et al. Application experience of Leica, Dako and Roche automated immunohistochemical staining platforms[J]. Journal of Clinical and Experimental Pathology, 2017, 33(4): 465-467. DOI:10.13315/j.cnki.cjcep.2017.04.031.
[4] 脱颖, 梁英杰. 探讨外院会诊玻片行全自动免疫组化仪染色异常的优化方法[J]. 临床与实验病理学杂志, 2021, 37(11): 1388-1389. DOI:10.13315/j.cnki.cjcep.2021.11.028.
Tuo Y, Liang YJ. Optimization method for abnormal staining of automated immunohistochemistry instrument in consultation slides from other hospitals[J]. Journal of Clinical and Experimental Pathology, 2021, 37(11):1388-1389. DOI:10.13315/j.cnki.cjcep.2021. 11.028.
[5] 陈东, 王伟. 国际标准的信息网络化建设在病理科实验室质量控制中的应用[J].诊断病理学杂志, 2013,20(4):193-195,250. DOI: 10.3969/j.issn.1007-8096.2013.04.01.
Chen D, Wang W. Application of international standard information network construction in quality control of pathology laboratory[J]. Chinese Journal of Diagnostic Pathology, 2013, 20(4): 193-195，250. DOI:10.3969/j.issn.1007-8096.2013.04.01.
[6] 步鹏. Ventana BenchMark XT全自动免疫组织化学仪操作流程的优化[J]. 实用医技杂志, 2016, 23(11):1241.
Bu P. Optimization of the operating procedure for Ventana BenchMark XT automatic immunohistochemistry instrument[J]. Journal of Practical Medical Techniques, 2016, 23(11): 1241.
[7] 陈刚, 宋国新, 韩雪, 等. 多类别阳性对照在免疫组化质控中的应用[J].诊断病理学杂志, 2024, 31(6): 585-586. DOI:10.3969/j.issn.1007-8096.2024.06.030.
Chen G, Song GX, Han X, et al. Application of multi-category positive controls in immunohistochemistry quality control[J]. Chinese Journal of Diagnostic Pathology, 2024, 31(6): 585-586. DOI:10.3969/j.issn. 1007-8096.2024.06.030.
[8] 陈杰伟, 刘君, 张淦梅, 等. 高效实用的组织芯片免疫组织化学对照体系的建立[J].中国组织化学与细胞化学杂志, 2017, 26(2):170-177.DOI:10.16705/j.cnki.1004-1850.2017.02.013.
Chen JW, Liu J, Zhang GM, et al. Establishment of an efficient and practical control system for tissue microarray immunohistochemistry[J]. Chinese Journal of Histochemistry and Cytochemistry, 2017, 26(2): 170-177. DOI:10.16705/j.cnki.1004-1850.2017.02.013.
[9] 商久妍, 贾珂琪, 郭启涛, 等. 乳腺癌HER-2表达异质性的研究进展及临床意义[J].临床与实验病理学杂志, 2023, 39(3): 339-342. DOI:10.13315/j.cnki.cjcep.2023.03.017.
Shang JY, Jia KQ, Guo QT, et al. Research progress and clinical significance of HER-2 expression heterogeneity in breast cancer[J]. Journal of Clinical and Experimental Pathology, 2023, 39(3): 339-342. DOI:10.13315/j.cnki.cjcep.2023.03.017.