大鼠颈上神经节切除对缓解面动脉痉挛作用的探讨

doi:10.13418/j.issn.1001-165x.2026.3.07

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中国临床解剖学杂志 ›› 2026, Vol. 44 ›› Issue (3) : 290-295. DOI: 10.13418/j.issn.1001-165x.2026.3.07

实验研究

大鼠颈上神经节切除对缓解面动脉痉挛作用的探讨

蔡梓立^1,2，任震宇^1,2，周树均³，庄跃宏³，吴吉文³*

作者信息 +

Effect of rat superior cervical ganglionectomy on relieving vasospasm in the facial artery area

Cai Zili^1,2, Ren Zhenyu^1,2, Zhou Shujun³, Zhuang Yuehong³, Wu Jiwen³*

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摘要

目的探讨颈上神经节切除对缓解面动脉区域血管痉挛及改善血流灌注的作用。方法将20只大鼠分成实验组10只与对照组10只。实验组大鼠行右侧颈上神经节切除，并对切除的标本进行免疫荧光染色。对照组行右侧假手术。构建血管痉挛模型：两组大鼠（n=20）分别于术后即刻与术后1 h进行机械诱导面动脉血管痉挛，分别在2个时刻采用激光散斑成像技术测量面动脉区域血流量及血管直径。实验组大鼠于术后1周分别接受Horner征评估、面动脉区域血流量及血管直径测量并同术后即刻痉挛前比较，最后分别从实验组、对照组取出面动脉标本，行免疫荧光染色。结果（1）术后即刻血管痉挛前、后，实验组同侧面动脉区域血流量及血管直径与对照组比较均无统计学差异（P>0.05）；（2）术后1 h血管痉挛前、后，实验组同侧面动脉区域血流量及血管直径均显著高于对照组（P<0.0001)；（3）实验组术后1周同侧面部均出现Horner征；同侧面动脉区域的血流量、血管直径均显著高于术后即刻痉挛前（P<0.0001）；免疫荧光结果示切除的标本中含有丰富的酪氨酸羟化酶(TH)阳性神经元。与对照组比较，实验组面动脉区域的TH阳性神经纤维密度显著降低。结论颈上神经节切除可缓解面动脉区域血管痉挛及改善血流灌注。

Abstract

Objective To investigate the effects of superior cervical ganglionectomy on relieving facial artery vasospasm and improving blood perfusion. Methods A total of 20 rats were randomly divided into an experimental group (n=10) and a control group (n=10). The experimental group underwent right superior cervical ganglionectomy, and the resected specimens were harvested to immunofluorescent staining. The control group received sham surgery. For the vasospasm model: mechanical induction of facial artery vasospasm was performed on rats from two groups (n=20) immediately after surgery and at 1h post-surgery. Laser speckle imaging was used to measure facial artery blood flow and vessel diameter at both time points. At 1w post-surgery, rats in the experimental group underwent Horner's syndrome evaluation, facial artery blood flow and diameter measurements, which were compared with pre-spasm measurements taken immediately after surgery. Finally, facial artery specimens were harvested from both groups for immunofluorescence staining. Results (1) Immediately after surgery, before and after vasospasm, there were no statistical differences in ipsilateral facial artery blood flow and vessel diameter between the experimental and control groups (P>0.05). (2) At 1h post-surgery, before and after vasospasm, the experimental group showed significantly higher ipsilateral facial artery blood flow and vessel diameter compared to the control group (P<0.0001). (3) At 1week post-surgery, all rats in the experimental group developed ipsilateral Horner's syndrome. Ipsilateral facial artery blood flow and vessel diameter were both significantly higher than pre-spasm measurements taken immediately after surgery (P<0.0001). Immunofluorescence results indicated that the resected specimens were rich in tyrosine hydroxylase (TH)-positive neurons. The density of TH-positive fibers in the facial artery region of the experimental group was significantly lower than that in the control group. Conclusions Superior cervical ganglionectomy can effectively relieve facial artery vasospasm and improve blood perfusion.

导出引用

蔡梓立, 任震宇, 周树均, 庄跃宏, 吴吉文. 大鼠颈上神经节切除对缓解面动脉痉挛作用的探讨[J]. 中国临床解剖学杂志. 2026, 44(3): 290-295 https://doi.org/10.13418/j.issn.1001-165x.2026.3.07

Cai Zili, Ren Zhenyu, Zhou Shujun, Zhuang Yuehong, Wu Jiwen. Effect of rat superior cervical ganglionectomy on relieving vasospasm in the facial artery area[J]. Chinese Journal of Clinical Anatomy. 2026, 44(3): 290-295 https://doi.org/10.13418/j.issn.1001-165x.2026.3.07

中图分类号： R322

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