丘脑网状核至海马不同亚区的神经通路

doi:10.13418/j.issn.1001-165x.2026.3.05

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中国临床解剖学杂志 ›› 2026, Vol. 44 ›› Issue (3) : 274-280. DOI: 10.13418/j.issn.1001-165x.2026.3.05

实验研究

丘脑网状核至海马不同亚区的神经通路

刘瑶¹，马文倩¹，黄海月¹，彭海蓉²，马畅³，张瑞¹，张莲香¹，文玉军¹*

作者信息 +

Neural pathways from the thalamic reticular nucleus to hippocampal subregions

Liu Yao¹，Ma Wenqian¹，Huang Haiyue¹，Peng Hairong²，Ma Chang³，Zhang Rui¹，Zhang Lianxiang¹，Wen Yujun¹*

Author information +

文章历史 +

摘要

目的明确丘脑网状核（TRN）至海马（HPC）不同亚区的神经通路。方法将顺行示踪剂生物素化葡聚糖胺（BDA）注射于小鼠脑内一侧TRN，在丘脑和海马观察TRN发出的顺行投射；将逆行示踪剂霍乱毒素B亚单位（CTb）注射于小鼠一侧海马的CA3区或齿状回（DG），在丘脑观察逆行标记的神经元胞体的分布。结果 BDA注射术后7 d，在同侧的丘脑前背侧核（AD）、丘脑前腹侧核（AV）及丘脑室旁核（PV）均可见BDA阳性神经纤维，其中投射至AD、AV的神经纤维表达囊泡谷氨酸转运体2（vGluT2），在海马DG区亦可见BDA阳性纤维；CTb注射术后7 d，在同侧AD、AV及PV均可见来自海马CA3区的CTb阳性神经元胞体，在同侧TRN可见来自DG区的CTb阳性神经元胞体。结论 TRN与海马CA3区存在间接通路，其投射路径为TRN→AD/AV/PV→CA3；TRN与海马DG区存在直接通路，TRN发出的神经纤维可直接投射于海马DG区。

Abstract

Objective To clarify the neural pathways from the thalamic reticular nucleus (TRN) to subregions of the hippocampus (HPC). Methods The anterograde tracer biotinylated dextran amine (BDA) was injected into the unilateral TRN of the mouse brain, and the anterograde projections originating from the TRN were observed in the thalamus and hippocampus. The retrograde tracer cholera toxin subunit B (CTb) was injected into the CA3 region or dentate gyrus (DG) of the unilateral hippocampus in mice, and the distribution of retrogradely labeled neuronal somata was examined in the thalamus. Results Seven days after BDA injection, BDA-positive nerve fibers were detected in the ipsilateral anterodorsal thalamic nucleus (AD), anteroventral thalamic nucleus (AV), and paraventricular thalamic nucleus (PV). Among these fibers, those projecting to the AD and AV expressed vesicular glutamate transporter 2 (vGluT2), and BDA-positive fibers were also observed in the hippocampal DG region. Seven days after CTb injection, CTb-positive neuronal somata originating from the hippocampal CA3 region were found in the ipsilateral AD, AV, and PV, while CTb-positive neuronal somata from the DG region were identified in the ipsilateral TRN. Conclusion An indirect pathway exists between the TRN and the hippocampal CA3 region, with the projection route being TRN→AD/AV/PV→CA3. A direct pathway is present between the TRN and the hippocampal DG region, where nerve fibers from the TRN directly project to the hippocampal DG region.

导出引用

刘瑶, 马文倩, 黄海月, 彭海蓉, 马畅, 张瑞, 张莲香, 文玉军. 丘脑网状核至海马不同亚区的神经通路[J]. 中国临床解剖学杂志. 2026, 44(3): 274-280 https://doi.org/10.13418/j.issn.1001-165x.2026.3.05

Liu Yao, Ma Wenqian, Huang Haiyue, Peng Hairong, Ma Chang, Zhang Rui, Zhang Lianxiang, Wen Yujun. Neural pathways from the thalamic reticular nucleus to hippocampal subregions[J]. Chinese Journal of Clinical Anatomy. 2026, 44(3): 274-280 https://doi.org/10.13418/j.issn.1001-165x.2026.3.05

中图分类号： Q189 R322.81

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