载脂蛋白C1过表达对肝癌耐药细胞HepG2/SR耐药性的影响

doi:10.13418/j.issn.1001-165x.2026.2.08

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中国临床解剖学杂志 ›› 2026, Vol. 44 ›› Issue (2) : 175-181. DOI: 10.13418/j.issn.1001-165x.2026.2.08

实验研究

载脂蛋白C1过表达对肝癌耐药细胞HepG2/SR耐药性的影响

陈亮¹，胡敏¹，赵颂^2,3，宋慧娟^2,3*

作者信息 +

Effect of apolipoprotein C1 overexpression on drug resistance of liver cancer drug resistant cell HepG2/SR

Chen Liang¹, Hu Min¹, Zhao Song^2,3, Song Huijuan^2,3*

Author information +

文章历史 +

摘要

目的探讨载脂蛋白C1（Apolipoprotein C1，APOC1）过表达对肝癌耐药细胞HepG2/SR耐药性的影响及可能机制。方法索拉非尼浓度递增法诱导肝癌HepG2细胞建立索拉非尼耐药细胞株HepG2/SR，将HepG2/SR细胞分为对照组和过表达APOC1组， CCK-8和EdU标记技术检测细胞增殖活性和增殖率，细胞划痕实验检测细胞迁移率，流式细胞术检测细胞凋亡率，Western blotting检测细胞中E-cadherin、N-cadherin和Vimentin蛋白表达水平。结果与对照组HepG2/SR细胞比较，APOC1 过表达组细胞增殖活性、增殖率和迁移率明显降低，细胞凋亡率明显升高，细胞中E-cadherin蛋白水平明显升高，N-cadherin和Vimentin蛋白水平明显降低。结论过表达APOC1可抑制HepG2/SR耐药细胞的增殖和迁移并促进细胞凋亡，其作用机制可能与抑制上皮-间质转化过程相关蛋白表达有关。

Abstract

Objective To investigate the effect of apolipoprotein C1 (APOC1) overexpression on drug resistance of liver cancer drug-resistant cells HepG2/SR and its possible mechanism. Methods The sorafenib resistant cells HepG2/SR were established by inducing the liver cancer HepG2 cells with a gradually increasing concentration of sorafenib. HepG2/SR cells were divided into a control and an APOC1 overexpression groups. CCK-8 assay and EdU labeling technique were used to detect cell proliferation activities and proliferation rates. Cell scratch assay was used to detect cell migration rates. Flow cytometry was used to detect cell apoptotic rates, and Western blotting to detect the expression levels of E-cadherin, N-cadherin and Vimentin proteins in the cells. Results Compared with the control cells, the proliferation activity, proliferation rate and migration rate of the HepG2/SR cells in APOC1 overexpression group were significantly decreased, the apoptotic rate of the cells was significantly increased, and the expression level of E-cadherin protein in the cells was significantly increased, while the expression levels of N-cadherin and Vimentin proteins were significantly decreased. Conclusions APOC1 overexpression can inhibit the proliferation, migration and promote the apoptosis of liver cancer drug resistant HepG2/SR cells. The mechanism may be related to the inhibition of protein expression involved in the epithelial-mesenchymal transition (EMT) process.

导出引用

陈亮, 胡敏, 赵颂, 宋慧娟. 载脂蛋白C1过表达对肝癌耐药细胞HepG2/SR耐药性的影响[J]. 中国临床解剖学杂志. 2026, 44(2): 175-181 https://doi.org/10.13418/j.issn.1001-165x.2026.2.08

Chen Liang, Hu Min, Zhao Song, Song Huijuan. Effect of apolipoprotein C1 overexpression on drug resistance of liver cancer drug resistant cell HepG2/SR [J]. Chinese Journal of Clinical Anatomy. 2026, 44(2): 175-181 https://doi.org/10.13418/j.issn.1001-165x.2026.2.08

中图分类号： R735.7

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