肺动脉高压中YTH结构域家族蛋白3的表达及临床价值分析

doi:10.13418/j.issn.1001-165x.2026.2.07

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中国临床解剖学杂志 ›› 2026, Vol. 44 ›› Issue (2) : 168-174. DOI: 10.13418/j.issn.1001-165x.2026.2.07

实验研究

肺动脉高压中YTH结构域家族蛋白3的表达及临床价值分析

乐田媛¹ ，李陈¹，汪馨²，罗涵深³，蒋丁胜²，李毅²*，方泽民²*

作者信息 +

Expression and potential clinical value of YTHDF3 in pulmonary hypertension

Yue Tianyuan¹, Li Chen¹, Wang Xin², Luo Hanshen³, Jiang Dingsheng², Li Yi²*, Fang Zemin²*

Author information +

文章历史 +

摘要

目的探究YTH结构域家族蛋白3（YTHDF3）在肺动脉高压（PH）中的作用及其潜在的临床意义。方法细胞荧光与Western blot检测YTHDF3在人肺动脉平滑肌细胞（HPASMCs）和PH患者肺组织中的表达水平；病理染色和组织荧光整体评估大鼠肺动脉管壁重塑程度与YTHDF3表达和定位；生信分析探索PH中YTHDF3下游调控通路。结果 YTHDF3在肺动脉高压患者肺动脉组织以及体外缺氧处理的HPASMCs中表达上调；随着PH模型大鼠的肺动脉血管重塑，YTHDF3在模型大鼠的肺动脉中层表达上调；人水平组学数据同样验证YTHDF3在PH组表达上调，并可能通过调控凋亡等相关通路影响肺动脉高压的发生发展。结论 YTHDF3富集于肺动脉高压重塑的肺动脉平滑肌中层，通过调节平滑肌细胞凋亡等通路参与肺动脉高压的发生和发展，具有相当的临床转化潜力。

Abstract

Objective To explore the expression of YTH domain-containing family protein 3 (YTHDF3) in pulmonary hypertension (PH) and evaluate its potential clinical significance. Methods The expression levels of YTHDF3 in human pulmonary arterial smooth muscle cells (HPASMCs) and lung tissues from patients with PH were detected using cellular immunofluorescence and Western blot analysis. Histopathological staining and tissue-wide fluorescence imaging was employed to evaluate the extent of pulmonary arterial wall remodeling and the expression and localization of YTHDF3 in rat models. Bioinformatic analysis was further conducted to explore the downstream regulatory pathways of YTHDF3 in PH. Results YTHDF3 expression upregulated in the pulmonary arterial tissues of patients with PH and in hypoxia-treated HPASMCs in vitro. More importantly, concomitant with the pathological remodeling of pulmonary arteries in a rat model of PH, YTHDF3 expression increased within the medial layer of these vessels. Consistent with these findings, human bulk omics data further confirmed the upregulation of YTHDF3 in PH samples and suggested its potential role in influencing the pathogenesis and progression of pulmonary hypertension, possibly through the regulation of apoptosis-related pathways. Conclusions YTHDF3 is enriched in the remodeled medial layer of pulmonary arteries in PH, where it contributes to the pathogenesis and progression of PH by regulating pathways such as smooth muscle cell apoptosis, demonstrating significant clinical application potential.

导出引用

乐田媛, 李陈, 汪馨, 罗涵深, 蒋丁胜, 李毅, 方泽民. 肺动脉高压中YTH结构域家族蛋白3的表达及临床价值分析[J]. 中国临床解剖学杂志. 2026, 44(2): 168-174 https://doi.org/10.13418/j.issn.1001-165x.2026.2.07

Yue Tianyuan, Li Chen, Wang Xin, Luo Hanshen, Jiang Dingsheng, Li Yi, Fang Zemin. Expression and potential clinical value of YTHDF3 in pulmonary hypertension [J]. Chinese Journal of Clinical Anatomy. 2026, 44(2): 168-174 https://doi.org/10.13418/j.issn.1001-165x.2026.2.07

中图分类号： R543.5

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