通过载褪黑素神经导管消除周围神经再生过程中的氧化应激

doi:10.13418/j.issn.1001-165x.2026.2.04

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中国临床解剖学杂志 ›› 2026, Vol. 44 ›› Issue (2) : 137-151. DOI: 10.13418/j.issn.1001-165x.2026.2.04

实验研究

通过载褪黑素神经导管消除周围神经再生过程中的氧化应激

李雪琪^1,2，杨佳伟^1,2，吴屹冰³，成晨⁴，张迟⁵，梅劲^1,2*，王宗焕¹*

作者信息 +

Eliminating oxidative stress during peripheral nerve regeneration with melatonin loaded nerve conduits

Li Xueqi^1,2, Yang Jiawei^1,2, Wu Yibing³, Cheng Chen⁴, Zhang Chi⁵, Mei Jin^1,2*, Wang Zonghuan¹*

Author information +

文章历史 +

摘要

目的开发一种具有载褪黑素的神经导管用于减少氧化应激对周围神经再生的阻碍。方法神经导管由干喷湿纺法进行加工，聚乳酸-羟基乙酸共聚物(PLGA)作为载药基质，褪黑素作为纺丝原液添加物一次成型。扫描电镜，傅立叶红外光谱，降解测试和药物释放测试用于表征神经导管的形态和物理性能。施万细胞与神经导管共培养用于测试神经导管的生物相容性。过氧化氢水溶液使施万细胞产生氧化应激状态，并用于测试神经导管对细胞的保护作用。SD大鼠坐骨神经修复实验验证神经导管的神经修复。结果干喷湿纺法成功制备含褪黑素神经导管。施万细胞在神经导管上的黏附以及增殖结果表明该神经导管具有优异的生物相容性。线粒体形态及JC-1膜电位检测显示神经导管对线粒体具有明显保护作用。q-PCR分析证实该神经导管在增强细胞增殖的同时，显著降低了细胞氧化应激水平并抑制了炎症反应。SD大鼠坐骨神经修复实验证明该神经导管对周围神经缺损具有明显的修复作用。结论通过干喷湿纺技术将褪黑素整合到神经导管中，使该导管具备多种功能，包括抗炎、抗氧化和神经保护特性。该方法有望为神经组织再生创造一种微环境构建方式，并为周围神经损伤的治疗提供新的方案。

Abstract

Objective Peripheral nerve injury leads to various degrees of functional defects. Nerve guidance conduits are considered as a new promising scaffold for peripheral nerve repair. However, conventional single-material nerve conduits have shown limited efficacy in protecting cells from posttraumatic inflammation. This study aims to develop a single-process PLGA-based nerve conduit loaded with melatonin to enhance the biological performance of pure PLGA materials by suppressing oxidative stress and inflammatory responses. Methods The PLGA conduit is prepared with dry-jet wet spinning methods. The melatonin is integrated into PLGA conduits directly with the single-step process. Scanning electrical microscope observation, FTIR test, degradation test and drug releasing test were performed to characterize the morphology and physical properties of the nerve conduits. Schwann cells were cultured to test the biocompatibility of the prepared nerve conduits. Oxidative stress was applied on Schwann cell using hydrogen peroxide. Then the protecting effects of the nerve conduits were tested on the hydrogen peroxide-treated cells. SD rat sciatic model was applied to test the conduit in vivo. Results The melatonin is successfully integrated into the nerve conduit with the dry-jet wet spinning method. Cell adhesion and proliferation test of the Schwann cell indicated that the nerve conduits exhibit excellent biocompatibility. While the mitochondrial morphology observation and JC-1 potential detection also showed protecting effects on Mitochondria. The q-PCR analysis showed nerve conduits reduced cellular oxidative stress and inflammatory responses while enhancing cellular proliferation. A marked enhancement on SD rat sciatic nerve regeneration was also observed on melatonin loaded conduits. Conclusions By integrating melatonin into PLGA using the dry-jet wet-spinning technique, the conduit is endowed with multiple functional advantages, including anti-inflammatory, antioxidant, and neuroprotective properties. This approach is expected to create a favorable microenvironment for nerve tissue regeneration and provide a new perspective for the treatment of peripheral nerve injuries.

导出引用

李雪琪, 杨佳伟, 吴屹冰, 成晨, 张迟, 梅劲, 王宗焕. 通过载褪黑素神经导管消除周围神经再生过程中的氧化应激[J]. 中国临床解剖学杂志. 2026, 44(2): 137-151 https://doi.org/10.13418/j.issn.1001-165x.2026.2.04

Li Xueqi, Yang Jiawei, Wu Yibing, Cheng Chen, Zhang Chi, Mei Jin, Wang Zonghuan. Eliminating oxidative stress during peripheral nerve regeneration with melatonin loaded nerve conduits[J]. Chinese Journal of Clinical Anatomy. 2026, 44(2): 137-151 https://doi.org/10.13418/j.issn.1001-165x.2026.2.04

中图分类号： R320.2720

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