目的 通过大鼠脑室造影明确其位置，为大鼠脑正电子发射计算机断层成像（PET）图像的解剖结构定位提供标志。 方法 成年大鼠在异氟烷麻醉下行示踪剂18F-FDG尾静脉注射，自由活动30 min后俯卧位固定于小动物PET扫描舱，PET/CT扫描并重建图像。扫描结束，异氟烷麻醉处死大鼠，经升主动脉灌注造影剂后再次行头部CT扫描，重建造影剂注射后的脑CT图像；用PMOD软件将PET和CT图像（第二次扫描）融合并观察脑室及相关结构的显影和PET图像的激活脑区的解剖定位。 结果 CT图像显示侧脑室、第三脑室、中脑导水管、第四脑室及脑室相关结构显影清晰；在PET-CT融合图像上，利用显影的脑室系统等结构可辨识出扣带回的A24a、A24a' 亚区、尾壳核、苍白球、伏隔核、丘脑带旁核等脑区，且大鼠脑PET图像的中线定位明确。 结论 清晰的脑室造影对大鼠脑PET图像的解剖定位起到重要的作用。
Abstract
Objective To locate of position of the ventricle system by rat ventriculography, which is used as anatomic localization mark to identify structure of positron emission tomography (PET) images of rat brain. Methods The rats were subjected to food deprivation prior to receiving tail vein injection of 18F-FDG. After an uptake period of 30 min, rats were placed in gantry and performed PET/CT scan in prone position. After the scan was completed, the rats were sacrificed under isoflurane anesthesia. A contrast agent was then perfused through the ascending aorta, and a head CT scan was performed again. The brain CT images after contrast agent injection were reconstructed. The PET and second scanned CT images were registrated and fused using PMOD software to observe the imaging of the ventricles and related structures, as well as the anatomical location of the activated brain regions in the PET images. Results The lateral ventricles, 3rd ventricle, aqueduct of midbrain and the 4th ventricle, the related structure to ventricles were clearly visualized in CT images. These structures could be used as landmark to locate the rat brain structure of PET images, such as A24a, A24a', caudate putamen, globus pallidus, accumbens nucleus, paratenial nucleus of thalamus. Conclusions Clear ventriculography plays a crucial role in the anatomical localization of PET images of the rat brain.
关键词
脑室造影； /
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PET； /
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CT； /
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脑； /
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大鼠
Key words
Ventriculography; /
PET; /
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CT; /
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Brain; /
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Rat
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基金
山东省重大科技攻关项目（2019JZZY021003）
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