内痔粘膜及血管上皮细胞VEGF/FGF2的表达与内痔分期的相关性分析

doi:10.13418/j.issn.1001-165x.2019.04.010

中国临床解剖学杂志 ›› 2019, Vol. 37 ›› Issue (4): 409-413.doi: 10.13418/j.issn.1001-165x.2019.04.010

内痔粘膜及血管上皮细胞VEGF/FGF2的表达与内痔分期的相关性分析

王琪¹，　经芳艳²，　邓永键¹

1.南方医科大学南方医院病理科/南方医科大学基础医学院病理学系；　2.南方医科大学南方医院肛肠科，广州 510515

收稿日期:2018-12-27 出版日期:2019-07-25 发布日期:2019-08-01
通讯作者: 邓永键，教授，博士生导师，E-mail：briandeng@163.com；经芳艳，硕士，主治医师，E-mail：stone1791@126.com
作者简介:王琪（1992-），在读硕士，研究方向：内痔的发病机制，E-mail：1597188080@qq.com
基金资助:
国家重点研发计划（973项目，2016YFC1201801）；国家自然科学基金（81702359，81672453）；广东省自然科学基金（2015A030310089）

Correlation analysis of VEGF/FGF2 expression in the hemorrhoidal mucosa and vascular epithelial cells with hemorrhoids staging

WANG Qi¹, JING Fang-yan², DENG Yong-jian¹

1. Department of Pathology, Nanfang Hospital/School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China；2. Department of proctology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China

Received:2018-12-27 Online:2019-07-25 Published:2019-08-01

摘要/Abstract

摘要： 目的通过观察人体内痔不同分期粘膜及血管内皮细胞生长因子（VEGF）及碱性成纤维细胞生长因子（FGF2）的表达，探讨内痔的发生及发展机制。方法收集南方医院肛肠科门诊手术切除的Ⅰ、Ⅱ、Ⅲ期内痔标本134例（Ⅰ期42例，Ⅱ期45例，Ⅲ期47例），内痔周围正常肠壁组织40例作为对照，采用HE染色观察组织的病理学变化，采用免疫组织化学方法检测血管内皮细胞VEGF及FGF2的表达。结果正常组及Ⅰ期内痔黏膜层被覆上皮完整，未见扩张血管；Ⅱ期内痔黏膜层被覆上皮破坏，黏膜肌层破坏，黏膜层内见新生血管；Ⅲ期内痔黏膜层被覆上皮破坏，见血管管壁增厚迂曲，管腔扩张；与正常粘膜成纤维细胞相比VEGF在粘膜层成纤维细胞表达水平明显升高，并随分期增高而增高（F=883.961，P<0.01），FGF2也存在相同表达（F=656.013，P<0.01）；与正常组相比VEGF在血管内皮细胞表达水平明显升高，并随分期增高而增高（F=776.561，P<0.01），FGF2在血管内皮细胞的表达水平存在相同趋势（F=1066.458，P＜0.01）。结论 VEGF及FGF2在内痔的形成过程中具有促进血管内皮细胞和粘膜下成纤维细胞增生的作用，同时可作为内痔发生发展的分子标志物。

关键词: 内痔, 　血管内皮生长因子, 　碱性成纤维细胞生长因子

Abstract: Objective　To investigate pathogenetic mechanism and progression of hemorrhoids at different stages by analyzing the expression of endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF2).　Methods　134 hemorrhoids specimen were collected from the out-patients of Nanfang Hospital, and there were 42 cases of hemarrhoids in gradeⅠ, 45 in grade Ⅱ, 47 in grade Ⅲ . Normal tissues of 40 cases among these patients were used as controls. Histopathological changes were obtained from HE stained slide of the disease tissues, as well as the expression of vascular endothelial cell growth factor (VEGF) and basic fibroblast growth factor (FGF2) with immunohistochemical staining.　Results　The epithelial cells were not injured and intact covering in the mucosa of the normal group and grade Ⅰ hemorrhoids, and no dilated blood vessels were found in these tissues. In grade Ⅱ hemorrhoids, they showed injury of the covering epithelial cells and mucosal muscularis, and neovascularization had been found in the mucosae propria. Grade Ⅲ hemorrhoids showed destructive cells of the covering epithelia in the mucosa, newly formed vessels with thickened wall, tortuous and dilated lumen in the mucosae propria. In comparison with the normal mucosa, expression of VEGF in fibroblasts was significantly increased along with hemarrhoids grading (F=883.961, P<0.01), and so did FGF2 (F=656.013, P<0.01); the same changes were observed with examination of VEGF (F=776.561, P<0.01) and FGF2 (F=1066.458, P<0.01) in vascular endothelial cells. Conclusion VEGF and FGF2 can promote the proliferation of vascular endothelial cells and fibroblasts in the formation of hemorrhoids, and can also be used as progression biomarkers of hemorrhoids.

Key words: Hemorrhoids, 　 Vascular endothelial growth factor, 　 Basic fibroblast growth factor

中图分类号:

R361.2

王琪, 经芳艳, 邓永键. 内痔粘膜及血管上皮细胞VEGF/FGF2的表达与内痔分期的相关性分析[J]. 中国临床解剖学杂志, 2019, 37(4): 409-413.

WANG Qi, JING Fang-yan , DENG Yong-jian . Correlation analysis of VEGF/FGF2 expression in the hemorrhoidal mucosa and vascular epithelial cells with hemorrhoids staging[J]. Chinese Journal of Clinical Anatomy, 2019, 37(4): 409-413.

参考文献 14

[1]	Riss S, Weiser FA, Schwameis K, et al. The prevalence of hemorrhoids in adults[J]. Int J Colorectal Dis, 2012, 27(2): 215-220.
[2]	王振军, 汤秀英, 王东, 等. 内痔的病理形态改变特征及其意义[J]. 中华外科杂志, 2006, 44(3): 177-180.
[3]	何有娣. FGF和VEGF在血管生成中的作用[J]. 国外医学药学分册, 2001, 28(6): 326-329.
[4]	Li SL, Jing FY, Ma LL, et al. Myofibrotic malformation vessels: unique angiodysplasia toward the progression of hemorrhoidal disease[J]. Drug Des Devel Ther, 2015, 13(9): 4649-4656.
[5]	Li D, Xie K, Zhang L, et al. Dual blockade of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2) exhibits potent anti-angiogenic effects[J]. Cancer Letters, 2016, 377(2): 164-173.
[6]	Cartland SP, Genner SW, Zahoor A, et al. Comparative evaluation of TRAIL, FGF-2 and VEGF-a-induced angiogenesis in vitro and in vivo[J]. Int J Mol Sci, 2016, 17(12): 2025.
[7]	Bai Y, Li P, Yin G, et al. BMP-2, VEGF and bFGF synergistically promote the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells[J]. Biotechnol Lett, 2013, 35 (3): 301-308.
[8]	Xiao L, Yang S, Hao J , et al. Endostar attenuates melanoma tumor growth via its interruption of b-FGF mediated angiogenesis[J]. Cancer Lett, 2015, 359(1): 148-154.
[9]	Tipoe G, Fung ML. Expression of HIF-1α, VEGF and VEGF receptors in the carotid body of chronically hypoxic rat[J]. Respir Physiol Neurobiol, 2003, 138(2-3): 143-154.
[10]	Eelen G, de Zeeuw P, Simons M, et al. Endothelial cell metabolism in normal and diseased vasculature[J]. Circ Res, 2015, 116(7): 1231-1244.
[11]	Yu P, Wilhelm K, Dubrac A, et al. FGF-dependent metabolic control of vascular development[J]. Nature, 2017, 545(7653): 224-228.
[12]	Wong CG, Taban M, Osann K, et al. Subchoroidal release of VEGF and bFGF produces choroidal neovascularization in rabbit[J]. Curr Eye Res, 2017, 42(2): 237-243.
[13]	Gurung HR, Carr MM, Bryant K, et al. Fibroblast growth factor-2 drives and maintains progressive corneal neovascularization following HSV-1 infection[J]. Mucosal Immunol, 2018, 11(1): 172-185.
[14]	Komekami Y, Konishi F, Makita K, et al. Rectal arterio-venous malformation (AVM) with bleeding of an internal hemorrhoid[J]. Clin J Gastroenterol, 2016, 9(1): 22-26.

内痔粘膜及血管上皮细胞VEGF/FGF2的表达与内痔分期的相关性分析

Correlation analysis of VEGF/FGF2 expression in the hemorrhoidal mucosa and vascular epithelial cells with hemorrhoids staging

可视化

摘要/Abstract

引用本文

使用本文

参考文献 14

相关文章 2

Metrics

本文评价

推荐阅读 0

[1]	张真翊, 陈曦, 王谦. 缺氧诱导因子1-血管内皮生长因子-Notch信号通路参与马蹄内翻足畸形的发生机制[J]. 中国临床解剖学杂志, 2022, 40(1): 62-66.
[2]	孙琦,单伟. 骨髓间充质干细胞和心脏Sca1 阳性细胞移植治疗心肌梗死的效果比较及其机制研究[J]. 中国临床解剖学杂志, 2019, 37(2): 160-164.