Chinese Journal of Clinical Anatomy >
The research of ovariectomy combined with glucocorticoid for induction of osteoporosis in rabbits
Received date: 2014-12-04
Online published: 2015-04-21
Objective To explore the effect of ovariectomy combined with glucocorticoid on rabbits bone metabolism and develop a new method for inducing osteoporosis in rabbits. Method 18 New Zealand female rabbits were randomly divided into 3 groups: Sham-operated group (SHAM, Physiological saline 5 ml·kg-1·d-1); ovariectomy group (OVX, Physiological saline 5 ml·kg-1·d-1); ovariectomy combined with glucocorticoid group (OVX +GC, Dexamethasone 1 mg·kg-1·d-1). The femoral bone mineral density (BMD) of the rabbit lumbar (3~6) and the right femoral were assessed at 0, 3 and 6-week, respectively. At the end of the experiment, the right femur biomechanics and the third lumbar vertebra bone tissue morphology metrology were determined. Result Compared with Sham-operated group, the BMD, the bone biomechanics (maximum load, fracture load) , the trabecular bone area percentage, the bone trabecular thickness and the bone trabecular number in the ovariectomy combined with glucocorticoid group were significantly decreased (P<0.05), the bone separating degree were significantly increased(P<0.05), while the ovariectomy group was not significantly different. Conclusion The method of ovariectomy combined with glucocorticoid is better at induction of osteoporosis in rabbits than ovariectomy alone and it is a feasible approach.
Key words： Ovariectomy; Glucocorticoid; Osteoporosis; Rabbits
ZHANG Yuan-Hua , SU Yan-Jie , HUANG Chao-Dong . The research of ovariectomy combined with glucocorticoid for induction of osteoporosis in rabbits[J]. Chinese Journal of Clinical Anatomy, 2015 , 33(2) : 204 -208 . DOI: 10.13418/j.issn.1001-165x.201
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