Chinese Journal of Clinical Anatomy >
Transcriptomic analysis of gene alteration related to the regulation of inflammation/immune responses after acute myoinjury
Received date: 2014-08-11
Online published: 2015-04-21
Objective To follow change of gene expression over time in crushed muscles of B6 mice using transcriptomic analysis. Methods Crush induced myoinjury was performed on gastrocnemius muscle of wide B6 mice. H&E and immunofluorecence staining were used to evaluate the necrosis and regeneration of damaged muscle. A mouse one color GE 4×44K G4846A, V2 microarray kit was used to perform global transcriptal expression analysis using muscle total RNA samples. Feature Extraction Software was used for the analysis of probe signals. Results Our microarray data identified 3494 genes (among 39429 probes) differentially expressed in crushed gastrocnemius muscle compared to normal muscle, and about 10% (319 probes) of these genes differed more than ten-fold in expression levels. In general, most of genes encoding proteins with important roles in muscle inflammation and immune up-regulated at day 2 and 4 postinjury, and then rapidly down-regulated and return to normal at day 10, which confirmed the effective but brief inflammatory response after acute myoinjury. 41 inflammatory/immune associated-genes persistently up-regulated at the day 7 and 10 after crush damage, which involved in many anti-inflammatory cytokines, chemokines and growth factor-related genes. Conclusions Transcriptome analysis indicates transient behavior of crush-induced muscle immune response. The persistently up-regulated genes maybe have anti-inflammatory effects during the muscle repair.
Key words： Crush; Inflammation; Genes; Transcriptome microarray analysis
YANG Tong-Qun , ZHANG Qian , GENG Chi-Lin , HU Xu-Chang , DING Meng-Cong , HUANG Mei-Xian , LIAO Hua-?? . Transcriptomic analysis of gene alteration related to the regulation of inflammation/immune responses after acute myoinjury[J]. Chinese Journal of Clinical Anatomy, 2015 , 33(2) : 171 -175 . DOI: 10.13418/j.issn.1001-165x.2015
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