Chinese Journal of Clinical Anatomy >
Effect of RGDyK peptide on the proliferation and migration of hepatic stellate cells
Received date: 2014-04-10
Online published: 2015-02-12
Objective To explore the effects of RGDyK cyclic peptide on αvβ3 integrin expression and cell migration in activated hepatic stellate cells. Methods Isolation and culture of primary hepatic stellate cell from Sprague-Dawley rat were performed and activated hepatic stellate cells model in vitro were established. RGDyK cyclic peptide was used to treat the activated hepatic stellate cells and MTT was adopted to calculate the growth inhibition rate of cells. Expression of of αvβ3 integrin expression in activated hepatic stellate cells was determined by immunochemistry staining and immunofluorescence staining , RT-PCR and Western Blot analysis of and compared between the control and treatment groups. observation the effects of cell migration was observed by wound scratche assay. Results RGDyK cyclic peptide inhibited activated hepatic stellate cells proliferation compared with the control group and a concentration-response relationship could be observed. RGDyK cyclic peptide inhibited the expression of αvβ3 integrin and a-SMA in the level of mRNA and protein in activated hepatic stellate cells respectively (P<0.05), and weakened the mobility of activated hepatic stellate cells (P<0.01). Conclusion RGDyK cyclic peptide can inhibitactivated hepatic stellate cell proliferation and migration by down-regulation of expression of αvβ3 integrin.
Key words： RGD cyclic peptide; Hepatic stellate cells; Integrin; Cell migration
GU Yan , SONG Zheng-Ji , FAN Gong , LI Yu-Lian , BANG Wei . Effect of RGDyK peptide on the proliferation and migration of hepatic stellate cells [J]. Chinese Journal of Clinical Anatomy, 2015 , 33(1) : 72 -75 . DOI: 10.13418/j.issn.1001-165x.201
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