Chinese Journal of Clinical Anatomy >
Three-dimensional porous scaffolds PLLA/n-HA in vitro biocompatibility studies
Objective To study the three-dimensional porous scaffolds of polylactic acid/nano- hydroxyapatite (PLLA/n-HA) in vitro biocompatibility, and to explore its suitability ascell culture materials and bone tissue engineering scaffolds. Methods The rat osteoblasts were seeded on PLLA/n-HA composite scaffolds after in vitro co-culture, CCK-8 assay was used to determine the rat osteoblast proliferation activity, inverted fluorescence microscope; scanning electron microscope were used to observe the PLLA/n- HA composite scaffold surface and the cell adhesion conditon within the pores. Results CCK-8 assay showed that the experimental group was not significantly different in proliferation of composite scaffolds cells with the control group (P>0.05); electron microscopy showed a large number of cell growth and adhesion in the composite scaffold surface and pores; and with the increase of culture time, the number of cells on material surface grew exponentially. Conclusion The three-dimensional porous scaffolds PLLA/n-HA have good biocompatibility and is expected to become a good performance of bone tissue engineering scaffolds.
Key words： Three-dimensional porous; PLLA; n-HA; Rat osteoblasts
HUANG Jiang-Hong , CHEN Ji-Lin , DUAN Chi , ZHANG Ju-Feng , XIONG Jian-Xi , SHU Wei-Min , WANG Da-Beng . Three-dimensional porous scaffolds PLLA/n-HA in vitro biocompatibility studies[J]. Chinese Journal of Clinical Anatomy, 2015 , 33(1) : 55 -59 . DOI: 10.13418/j.issn.1001-165x.201
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