Chinese Journal of Clinical Anatomy >
Preparation and hemocompatibility study of carboxyl single-walled carbon nanotubes
Received date: 2014-08-04
Online published: 2015-02-12
Objective In this study, carboxyl single-walled carbon nanotubes (SWCNT-COOH) were prepared, and their hemocompatibility studied. Methods SWCNT was treated with H2SO4/HNO3 mixture. The introduction of carboxyl groups on SWCNT was confirmed with Fourier transform infrared spectroscopy (FTIR) and zeta potential analyzer. The effect of SWCNT and SWCNT-COOH on red blood cell morphology and blood coagulation was studied by scanning electron microscope (TEM) observation and thromboelastography (TEG) assay, respectively. Results SWCNT-COOH was successfully synthesized as confirmed by FITC and zeta potential measurement. SWCNT-COOH at 10 mg/ml caused aggregation and morphological alteration of red blood cells. SWCNT at 0.01 mg/ml and SWCNT-COOH at 0.001 mg/ml promoted the activity of clotting factors. Conclusion SWCNT-COOH can be successfully prepared by oxidation with H2SO4/HNO3 mixture. The information on the effect of SWCNT and SWCNT-COOH on red blood cell aggregation and morphological change, and on blood coagulation is important for their design and biomedical applications.
LIN Jian-Sheng , DIAO Ting-Ting , SONG Xiao-Ping , TU Lei , QIU Xiao-Zhong . Preparation and hemocompatibility study of carboxyl single-walled carbon nanotubes[J]. Chinese Journal of Clinical Anatomy, 2015 , 33(1) : 51 -54 . DOI: 10.13418/j.issn.1001-165x.2015
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