Chinese Journal of Clinical Anatomy >
Effect of the exosomes from marrow-derived cardiac stem cells on apoptosis in the ischemia and hypoxia injured myocardial cells from adult rats
Received date: 2014-07-09
Online published: 2014-12-04
Objective To observe the apoptosis of injured adult rats myocardial cells induced by ischemia and hypoxia in vitro and the biological functions of the exosomes from marrow-derived cardiac stem cells (MCSCs-exosomes)．Methods Cultured adult rats myocardial cells were divided into the control group，the ischemia-hypoxia group and the MCSCs-exosomes pretreated group． In vitro，we counted the cells and detected the expression of Bcl-2，bax by Western Blotting．The cell cycles and apoptosis were measured by flow cytometry． Results Compared with the control group，the model group showed lower cell counts(P<0.01) and higher apoptosis ratio． We also observed that the expression of Bcl-2 / bax was decreased(P<0.01) and the cell cycle was blocked at S phase．Compared with the ischemia-hypoxia group，MCSCs-exosomes pretreated groups showed apparent tolerance to ischemic and hypoxic injury in vitro，which manifested a higher cell viability，lower apoptosis ratio，increased expression of Bcl-2/bax and relief of the block of S phase( all P<0.05)． Conclusions Ischemia and hypoxia could cause the apoptosis of cells in vitro.MCSCs-exosomes exhibited anti-apoptosis ability in vitro．All these may provide experimental evidences for the mechanisms of MCSCs-exosomes in therapy of tissue injury．
CUI Xiao-Jun , ZHANG Jian-Kai , ZHANG Zhi , ZHOU Li-Na , GUO Jin-Hua , WANG Xiao-Gong , MA Ying-La . Effect of the exosomes from marrow-derived cardiac stem cells on apoptosis in the ischemia and hypoxia injured myocardial cells from adult rats[J]. Chinese Journal of Clinical Anatomy, 2014 , 32(6) : 685 -689 . DOI: 10.13418/j.issn.1001-165x.2014
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