Chinese Journal of Clinical Anatomy >
The regeneration of an acellular ureter transplanted to the bladder
Received date: 2014-04-11
Online published: 2014-06-04
Objective After decellularization, a rat’s ureter attached to a piece of bladder wall was transplanted to another rat’s bladder wall. Without reseeding any cells, the body itself was to reconstruct the ureter. Methods 20 SD rats’ ureter were decellularized by Triton-X100 and SDS followed by sterilization, after authentication of the structure of the scaffolds we transplanted it onto another rat’s bladder wall with ligation of the distal part of the ureter. After 1-4 weeks, we sacrificed those rats and processed the transplanted structures with HE stain and immunofluorescence for observation and analysis. Results We divided the ureters into three parts the part attached to the bladder(B)、the middle part(M)、the freeend (D). After 4 weeks , the epithelial cells and smooth muscles cells could be found in B part, fat cells in M part and fibrosis in D part. Particularly, after 1-2weeks, there are epithelial cells regrown in B part and muscle cells regrown after 4 weeks. Conclusion It is possible that ureter scaffolds can regenerate in body without reseeding and the epithelial layer would regrow first before muscular layer .
Key words： Tissue scaffolds; Regeneration; Ureter; Immunogenicity
SHAO Ying-Kuan , LIN Ke-Zhi , TU Ya-Ling , RAO Zhi-Heng , LI Re-Bing , MEI Jin . The regeneration of an acellular ureter transplanted to the bladder[J]. Chinese Journal of Clinical Anatomy, 2014 , 32(3) : 330 -334 . DOI: 10.13418/j.issn.1001-165x.2014
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