Chinese Journal of Clinical Anatomy >
PNGF conduit seeded with bone marrow derived mesenchymal stem cells to repair 12-mm sciatic nerve defect of rat
Received date: 2014-03-06
Online published: 2014-06-04
Objective To evaluate the effectiveness of tissue-engineered artificial nerve consists of novel arginine-glycine-aspatic acid (RGD) peptide modification of poly{(lactic acid)-co-[(glycolic acid)-alt-(L-lysine)]}/poly(d,l-lactic acid)/β-tricalcium phosphate/nerve growth factor(PRGD/PDLLA/β-TCP/NGF,PNGF) sustaining release conduit seeded with Bone marrow derived mesenchymal stem cells(BMSCs) in bridging 12-mm sciatic nerve defect in rats. Methods 30 isogenic adult male Wister rats were randomly divided into 3 groups (n=10 in each), the left sciatic nerve was exposed to create a 12-mm gap in all rats. Animals in the three groups were bridged with PNGF conduits (A), PNGF conduits filled with BMSCs(B) and autografts, respectively. The left was the experimental side and the right was the normal, intact control. At three months after the operation, recovery rate of wet weight of triceps suraes, histological observation of regenerative nerve and target muscle tissue were performed to evaluate the functional recovery of sciatic nerve. Results The conduit wall became thinner three months after the operation, continuously regenerated nerve tissue was located in the conduit, and the surface of regenerated nerve can be observed with creeping growth of nutrient vessels. All regenerated nerves were thinner than normal, intact nerves. The result of SFI in Group B was superior to that of Group A, and the difference was statistically significant(P＜0.05); the recovery rate of nerve conduction velocity, the recovery rate of weight triceps surae, the number of myelinated nerve fibers and thickness of myelin sheath in Group B were significantly higher than those in Group A(P＜0.01), acquiring similar results to that of autologous nerve transplantation group. Conclusion PRGD/PDLLA/β-TCP/NGF composite nerve conduit seeded with Bone marrow derived mesenchymal stem cells can effectively promote nerve regeneration after bridging sciatic nerve defect in rats. The effect was as good as that of autograft.
ZHANG Wei-Cai , HUANG Ji-Feng , YAN Qiong-Jiao , LI Shi-Pu . PNGF conduit seeded with bone marrow derived mesenchymal stem cells to repair 12-mm sciatic nerve defect of rat[J]. Chinese Journal of Clinical Anatomy, 2014 , 32(3) : 325 -329 . DOI: 10.13418/j.issn.1001-165x.2014
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