Chinese Journal of Clinical Anatomy >
The effect of NF-κB signaling pathway on iNOS expression in BLP-tolerized macrophages
Received date: 2014-03-26
Online published: 2014-06-04
Objective In order to explore mechanisms underlying enhanced bacterial clearance of BLP-tolerized macrophages，we detected the expression of inducible nitric oxide synthase (iNOS) and investigated whether this expression was regulated by NF-κB signaling pathway in BLP-tolerized macrophages to bacterial infection. Methods Through comparison of the phagocytosis and intracellular bacterial killing of E.coli between Naive and BLP-tolerized mice bone marrow-derived macrophages (BMMs), we evaluated the bacterial clearing capability of BLP-tolerized macrophages. Next, the mRNA level of iNOS in BLP-tolerized macrophages was detected by real-time PCR, and the translocations of p65 from cytoplasm to nucleus were shown by immunofluorescence.Finally, the activation of NF-κB signaling pathway was inhibited and the mRNA expression of iNOS in BLP-tolerized BMMs were observed. Results Compared to Naive macrophages, the phagocytosis and intracellular bacterial killing of BLP-tolerized macrophages were significantly enhanced (P<0.05). The mRNA level of iNOS in BLP-tolerized macrophages was significantly increased (P<0.05), which could be significantly affected when the activation of NF-κB signaling pathway was inhibited (P<0.05). Conclusion The study suggests that iNOS expression increases through the activation of NF-κB signaling pathway in BLP-tolerized macrophages to bacterial infection.
Key words： BLP tolerance; Bacterial phagocytosis; iNOS; NF-κB
LI Xue , WANG Xi-Gan , LUO Hai-Hua , ZHONG Yu-Yun , LEI Ye-Ming , LEI Shan , CA Jun-Wei , JIANG Yong , LIU Jing-Hua . The effect of NF-κB signaling pathway on iNOS expression in BLP-tolerized macrophages[J]. Chinese Journal of Clinical Anatomy, 2014 , 32(3) : 300 -305 . DOI: 10.13418/j.issn.1001-165x.2014
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