Chinese Journal of Clinical Anatomy >
Notexin effects on the survival and proliferation of CD8+ T cell in the injuried-muscle draining lymph nodes
Received date: 2014-02-23
Online published: 2014-06-04
Objective To explore the effects of intramuscular injection of Notexin on transferred OT-I cells in muscle draining lymph nodes of C57BL/6 mice. Methods Tibialis anterior（TA）injury were performed by intramuscular injection of Notexin, or crush skill on B6 mice, separately. CFSE-labeled OT-I cells were adoptively transferred to myoinjury mice, and followed by OVA i.m. injection. TA draining lymph nodes were received, and lymphocytes were collected and analyzed the proliferation of OT-I cells by CFSE fluorescence intensity evaluation using flow cytometry. Activated spleen cDC cells were collected and purified from one B6 mouse received OVA i.v. injection. Purified cDC cells were co-cultured with CFSE-labeled OT-I cells. Notexin with different concentration were added into co-culturing system. After 72h, the survival and proliferation of OT-I cells were detected. Results According to the detection results of CFSE fluorescence intensity, OT-I cells in crush induced-damaged muscle draining lymph nodes were activated and proliferated rapidly under the stimulation of OVA antigen. For Notexin injection-induced muscle damage, no cell proliferation of OT-I cells were detected in TA draining lymph nodes at the day 4, but the proliferation of OT-I cells recovered at the day 7. Co-culturing with activated cDCs cells induced a obvious proliferation of OT-I cells in vitro, however, OT-I cells lost the proliferation ability if the co-culturing system were added with Noteixn, even if the concentration of Notexin was very lower（1：1000）. Conclusions The activity and proliferation of CD8+ T cells will be interfered by intramuscular injection of Notxin. Therefore, we suggest that Notexin injection is unsuitable for investigating T cell response associated with myoinjury.
Key words： Notexin; Adoptive transfer; OT-I cell; Myoinjury
MA Beng , SHU Rong , LIU Nie-Hui , SHI Dan-Dan , CENG Hui-Jun , CAO Biao , LIAO Hua . Notexin effects on the survival and proliferation of CD8+ T cell in the injuried-muscle draining lymph nodes[J]. Chinese Journal of Clinical Anatomy, 2014 , 32(3) : 296 -299 . DOI: 10.13418/j.issn.1001-165x.2014
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