Chinese Journal of Clinical Anatomy >
Relationship between olfactory function and the number of mature olfactory neurons in olfactory epithelium of the adult mouse
Received date: 2013-08-30
Online published: 2014-04-21
Objective To explore the sufficient amount of mature olfactory receptor neurons(ORNs) in olfactory epithelium for maintaining normal olfactory function and to investigate the relationship between a simple olfactory test outcome and the mature olfactory neuronal population. Methods The anosmia model was induced in 8- to 10-week-old mice by intranasal irrigation with 0.7% Triton X-100 . The“buried food pellet” test was conducted at 3, 7, 21, 49, 56 days a after the anosmia model was established. The olfactory marker protein(OMP) olfactory epithelia(OE) was detected with immuno?uorescence to analyze the relationship“buried food pellet” test outcome and the mature olfactory neuronal population of OE. Results Olfactory marker protein(OMP) was physiologically expressed in mature olfacotry receptor neurons(ORNs) body, dendrites, and submucosa axons bundles. At 3rd,7th day after nose-irrigation with TritonX-100, time spend to find food was prolonged obviously(F =32.04，P <0.01)and was longer than that at 21th,49th,56th and control group when compared within each two groups with Bonferroni methode(P <0.01).The mature ORNs in OE was minimal at 3rd,7th after treatment (F =223.97, P <0.001). The food-?nding time was signi?cantly correlated with the number of mature ORNs in OE(r =－0.757, P <0.001).The anosmia mice begin to recover at 21th day after nasal irrigation, when the OMP(+) recovered to 28.66%. Conclusions Our studies confirmed that mice olfactory function is signi?cantly correlated with the number of mature ORNs in OE. The adult mice with anosmia could regain olfactory function when the mature ORNs in OE recovered to 28.66% of the control group.
Key words： Dysosmia; Olfactory; OMP; TritonX-100
CENG Xian-Beng , CHEN Ge-Xin , FU Qing-Ling , XU Rui , WEN Wei-Beng , SHI Jian-Bei , HU Geng . Relationship between olfactory function and the number of mature olfactory neurons in olfactory epithelium of the adult mouse[J]. Chinese Journal of Clinical Anatomy, 2014 , 32(2) : 192 -195 . DOI: 10.13418/j.issn.1001-165x.2014
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