Chinese Journal of Clinical Anatomy >
BMP-2 induced up-regulation of RBP4 that is a positive regulator of osteoblast differentiation
Received date: 2013-09-16
Online published: 2014-04-21
Objective To explore the function of retinol binding protein 4 (RBP4) on BMP-2 induced BMSCs osteogenic differentiation in mice. Methods RBP4 expression was detected by western-blot and qPCR during osteogenic differentiation of BMSCs induced by rhBMP-2. Osteogenic differentiation of mouse BMSCs was detected after RBP4 down-expressed by RNA interference. Results Osteogenic differentiation of BMSCs was significant after being induced by rhBMP-2 cells for 7 d, with RBP4 increasing significantly. Mice BMSCs osteogenic differentiation was significantly inhibited after RBP4 siRNA transfection for 24 h followed with rhBMP-2 inducing for 7 d. Conclusions BMP-2 induced up-regulation of RBP4 is a positive regulator of osteoblast differentiation.
Key words： BMSCs; BMP; RBP4; Osteogenesis
JU Hua-Jun , HONG Jin-Sheng , ZUO Cuan , MI Hai-Feng . BMP-2 induced up-regulation of RBP4 that is a positive regulator of osteoblast differentiation[J]. Chinese Journal of Clinical Anatomy, 2014 , 32(2) : 184 -188 . DOI: 10.13418/j.issn.1001-165x.2014
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