Chinese Journal of Clinical Anatomy >
Effects of contusion velocity on the cervical primary spinal cord injury in rats
Received date: 2013-09-17
Online published: 2014-04-21
Objective To verify the contusion velocity as a factor of spinal cord injury and determine cervical primary spinal cord injury in rats at different contusion speed. Methods Twenty adult and male Sprague-Dawley rats were divided randomly into the fast group (500 mm/s, n=8), the slow group (5 mm/s, n=8) and the sham control group (n=4). The contusion tip was a cone-shape flat head with a diameter of 4 mm, and driven by a servo-electromagnetic material testing machine to perform a 1.5 mm contusion at the dorsal cord of C5. All rats were perfused intracardially right after injury. The spinal cord sample with 15 mm in length and the epicenter in the middle was sectioned for histological test. Serial parasagittal sections of the spinal cord were cut and then stained with Hematoxylin and Eosin for inspection of the gross morphology and quantification of hemorrhage volume. The axonal injury was detected with β-amyloid precursor protein by immunohistochemistry. Results Hemorrhage was observed on all spinal cords after contusion. The hemorrhage looked darker in the fast group. The contusion displacement for the fast and slow contusion groups was (1.50±0.05) mm and (1.51±0.04) mm, and the maximal force (5.3±1.2) N and (2.8±0.6) N, respectively. There was significant difference in the maximal force between the groups (P=0.001). The hemorrhage was mainly located in the gray matter and few in the white matter. Total hemorrhage, the hemorrhage in the gray and white matter were 0.94 mm3, 0.71 mm3 and 0.23 mm3 for the fast group, and 0.55 mm3, 0.43 mm3 and 0.12 mm3 in the slow group, respectively. There was significant difference in total hemorrhage and hemorrhage in the gray matter between two contusion groups (P＜0.05). Axonal disruption seemed more serious after contusion at higher speed. Conclusion The present study suggested that the contusion velocity is a factor of primary spinal cord injury. Contusion at higher speed results in severer primary spinal cord injury, more hemorrhage and axonal disruption.
Key words： Contusion; Velocity; Primary; Spinal cord injury; Rat
ZHOU Jian , WANG Xiao-Meng , LIU Qi , JIANG Jie , HUANG Zhi-Beng , TUN Xiu-Hua , SHU Jing-An . Effects of contusion velocity on the cervical primary spinal cord injury in rats[J]. Chinese Journal of Clinical Anatomy, 2014 , 32(2) : 179 -183 . DOI: 10.13418/j.issn.1001-165x.2014
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