Chinese Journal of Clinical Anatomy >
Experimental study of ZHX3 gene transfected BMSCs and osteogenesis in vitro
Received date: 2013-11-14
Online published: 2014-04-21
Objective To investigate the influence of zinc fingers and homeoboxes 3 gene (ZHX3) transfected BMSCs and osteogenesis induced in vitro. Method Transfect the rat BMSCs with lentivirus overexpressing of ZHX3 genet. At the same time, BMSCs transfected with no-load virus serves as the negative control group and BMSCs without any treatment as the blank control group. Fluorescent microscope were used to count cell number and calculate the rate of transfection. Western blot was used to detect the protein expression of ZHX3 gene in the transected cells. The cells received In-vitro inducement of for 21d, after which alkaline phosphates staining and alizarin red staining were performed to observe osteogenic ability. Results (1) Cells express BMSCs phenotype could be obtained by adherent method.(2) After transfection, the ZHX3 gene overexpression group and the negative control group both expressed green fluorescence, blank control group do not express, and ZHX3 gene expression in overexpression group is higher than the negative control group.(3) cells in each group were found to be positive with expression of alkaline phosphatase. Kaplow show that the score of overexpression group is higher than the other two groups( P≤0.05 ). And mineralized nodules were visible among all groups, But the number and volume of mineralized nodules in overexpression group was larger than the others. Conclusion ZHX3 gene may promote BMSCs in vitro osteogenesis.
Key words： ZHX3 gene; Cell transfection; BMSCs; Osteogenic
LI Ji , QIN Shu-Jian , BAO Cui-Fen , SANG Yao-Cai , JUE Hui-Ying . Experimental study of ZHX3 gene transfected BMSCs and osteogenesis in vitro[J]. Chinese Journal of Clinical Anatomy, 2014 , 32(2) : 174 -178 . DOI: 10.13418/j.issn.1001-165x.201
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