Chinese Journal of Clinical Anatomy >
Effect of different hydroxyapatite shapes on osteoclastogenesis of RAW264.7 cells in vitro
Received date: 2014-01-10
Online published: 2014-04-21
Objective To investigate the effect of different hydroxyapatite shapes on osteoclastogenesis of RAW264.7cells. Methods Hydroxyapatite with different shapes was synthesized through a hydrothermal treatment with further pH adjustments, and characterized by filed scanning electron microscope(SEM).Live/Dead staining was performed to evaluate the cell viability of different hydroxyapatites co-cultured with RAW264.7 cells. Tartrate-resistant acid phosphatase (TRAP) staining and quantitative RT-PCR (qRT-PCR) analysis were used to identify osteoclastogenesis and investigate transcriptional changes of osteoclastogenesis-related genes, under the stimulation of the receptor activator of nuclear factor-κB ligand (RANKL) in vitro. Results Live/Dead staining validated that the microrod and microsphere HAp particles were biocompatible and no difference of cell viability was detected between two particles after 24h co-culture. The qRT-PCR results showed that the two shaped HAp particles significantly inhibited the RANKL-induced osteoclastogenesis of RAW264.7 cells, especially for micrord particles compared to that of microsphere ones. Conclusions Hydroxyapatites with different shapes could initiate different degree on osteoclastogenesis of RAW264.7cells.
Key words： Hydroxyapatite; Shape; RAW264.7; Osteoclastogenesis
CENG Hui-Jun , CAO Biao , LIU Nie-Hui , CHEN Rong , SHI Dan-Dan , SHU Rong , OU Yang-Jun , LIAO Hua . Effect of different hydroxyapatite shapes on osteoclastogenesis of RAW264.7 cells in vitro[J]. Chinese Journal of Clinical Anatomy, 2014 , 32(2) : 170 -173 . DOI: 10.13418/j.issn.1001-165x.2014
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