Chinese Journal of Clinical Anatomy >
A novel quantitative ultrasound technique for osteogenesis evaluation of the injectable chitosan/nano-hydroxyapatite/ collagen scaffold
Received date: 2013-05-15
Online published: 2014-04-21
Objective An injectable scaffold based on nano-hydroxyapatite (nHA), collagen (Col) and chitosan (CS) was synthesized in a preliminary study. In this study, we proposed a novel quantitative noninvasive technique for osteogenesis evaluation of the scaffold. Methods The CS and CS/nHAC scaffold was respectively subcutaneously injected into Sprague-Dawley rats mediated by ultrasound. After 1h, 14d and 28d, the gray-scale value, volume and blood flow of the scaffold were measured using diagnostic technique software. The animals were evaluated by type-B ultrasonic and color doppler ultrasonography. Results Under mediation of ultrasound, the scaffold was accurately injected in. With evaluation of 28 days, we found the ROI gray-value of CS/nHAC was increasingly faster than CS/nHAC and the volume of CS/nHAC reduced slower. Conclusions CS/nHAC is more appropriate for fracture repairing than CS. And the ultrasound is proved a novel noninvasive technique for osteogenesis evaluation.
Key words： Injectable scaffold; Ultrasound; Noninvasive
YAN Yu-Ting , LI He , YUAN Ru , LI Song-Jian , CHEN Pan . A novel quantitative ultrasound technique for osteogenesis evaluation of the injectable chitosan/nano-hydroxyapatite/ collagen scaffold[J]. Chinese Journal of Clinical Anatomy, 2014 , 32(2) : 162 -166 . DOI: 10.13418/j.issn.1001-165x.2014
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