Effect of apolipoprotein C1 overexpression on drug resistance of liver cancer drug resistant cell HepG2/SR&nbsp;

Chen Liang, Hu Min, Zhao Song, Song Huijuan

doi:10.13418/j.issn.1001-165x.2026.2.08

Chinese Journal of Clinical Anatomy >

2026 , Vol. 44 >Issue 2: 175 - 181

DOI: https://doi.org/10.13418/j.issn.1001-165x.2026.2.08

Effect of apolipoprotein C1 overexpression on drug resistance of liver cancer drug resistant cell HepG2/SR

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1. Department of Cellular Biology and Genetics，School of Basic Medical Sciences, Jinzhou Medical University, Jinzhou 121000，China; 2. Collaborative Innovation Center for Age-related Disease, Life Science Institute of Jinzhou Medical University, Jinzhou 121000, China; 3. High Educational Key Laboratory of Molecular Cell Biology and New Drug Research in Liaoning Province, Jinzhou Medical University, Jinzhou 121000, China

Received date: 2025-05-06

Online published: 2026-04-16

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Abstract

Objective To investigate the effect of apolipoprotein C1 (APOC1) overexpression on drug resistance of liver cancer drug-resistant cells HepG2/SR and its possible mechanism. Methods The sorafenib resistant cells HepG2/SR were established by inducing the liver cancer HepG2 cells with a gradually increasing concentration of sorafenib. HepG2/SR cells were divided into a control and an APOC1 overexpression groups. CCK-8 assay and EdU labeling technique were used to detect cell proliferation activities and proliferation rates. Cell scratch assay was used to detect cell migration rates. Flow cytometry was used to detect cell apoptotic rates, and Western blotting to detect the expression levels of E-cadherin, N-cadherin and Vimentin proteins in the cells. Results Compared with the control cells, the proliferation activity, proliferation rate and migration rate of the HepG2/SR cells in APOC1 overexpression group were significantly decreased, the apoptotic rate of the cells was significantly increased, and the expression level of E-cadherin protein in the cells was significantly increased, while the expression levels of N-cadherin and Vimentin proteins were significantly decreased. Conclusions APOC1 overexpression can inhibit the proliferation, migration and promote the apoptosis of liver cancer drug resistant HepG2/SR cells. The mechanism may be related to the inhibition of protein expression involved in the epithelial-mesenchymal transition (EMT) process.

Key words： Apolipoprotein C1; ; ; Liver cancer; ; ; Sorafenib resistance; ; ; Epithelial-mesenchymal transition

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Chen Liang, Hu Min, Zhao Song, Song Huijuan . Effect of apolipoprotein C1 overexpression on drug resistance of liver cancer drug resistant cell HepG2/SR [J]. Chinese Journal of Clinical Anatomy, 2026 , 44(2) : 175 -181 . DOI: 10.13418/j.issn.1001-165x.2026.2.08

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