Objective To investigate the localization and regulation of cytoskeleton-related proteins PDLIM5 in human fibroblasts． Methods After the expression of PDLIM5 was down-regulated by lentivirus transfection in human fibroblasts, CCK8 cell proliferation assay, cell scratch assay and Transwell migration assay were performed to detect the effect of PDLIM5 on cell proliferation and migration ability. Immunofluorescence staining was used to observe the cellular localization of PDLIM5. Results Down-regulating of PDLIM5 expression inhibited, the proliferation and migration ability of human fibroblasts. Immunofluorescence staining showed that PDLIM5 was mainly expressed in the cytoplasm and localized in the microfilament. Conclusions PDLIM5 is localized in cytoskeleton microfilaments and down-regulation of PDLIM5 expression can inhibit cell proliferation and migration ability of human fibroblasts.
Huang Xiaolan, Peng Yan, Yang Yuchao, Fan Tingyu, Sun Bing, Asmat Ullah Khan, Dai Jingxing, Ouyang Jun, Zhong Shizhen
. Localization and regulation of cytoskeleton associated proteins PDLIM5 in human fibroblasts[J]. Chinese Journal of Clinical Anatomy, 2022
, 40(5)
: 536
-540
.
DOI: 10.13418/j.issn.1001-165x.2022.5.07
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