Objective To investigate the immunoreactivity induced by SOMNP, CDMNP and CDMNP-PEG-CD magnetic nanoparticles implanted into mouse skeletal muscle. Methods SOMNP was prepared by compounding magnetic Fe3O4 and SiO2. Polyethylene glycol (PEG) and cyclodextrin (β-CD) molecules were linked to SOMNP to synthesize CDMNP, and polypseudorotaxanes were further added to synthesize CDMNP-PEG-CD magnetic nanoparticles. particles. Three kinds of nanoparticles were placed into the gastrocnemius muscle of B6 mice, respectively. Histochemical staining, immunofluorescence and FACS analysis were used to evaluate the characteristics of myotoxicity and intramuscular inflammatory exudation induced by SOMNP, CDMNP and CDMNP-PEG-CD nanoparticles at different implantation periods. Results Linking of SOMNP to β-CD, PEG/β-CD and polypseudorotaxanes will limit the intramuscular diffusion of nanoparticles, resulting in prolonged intramuscular residence time of CDMNP and CDMNP-PEG-CD, myocyte necrosis in the vicinity of the material, intramuscular mononuclear/Macrophage aggregation. Compared with CDMNP-PEG-CD, CDMNP nanoparticles attracted more T cells into muscle tissue. Conclusion SOMNP, CDMNP and CDMNP-PEG-CD nanoparticles are all potential immune inducers in vivo. The modification of polypseudorotaxanes endowed CDMNP-PEG-CD nanoparticles with better in vivo compatibility.
Jian Xiaoting, Wan Han, Huang Jingwen, Lan Haiqiang, Liao Zhaohong, Ke Yu, Liao Hua, Zhu Chunmin
. Analysis of skeletal muscle reactivity induced by CDMNP-PEG-CD magnetic nanoparticles[J]. Chinese Journal of Clinical Anatomy, 2022
, 40(2)
: 173
-180
.
DOI: 10.13418/j.issn.1001-165x.2022.2.11
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