Objective To explore the possibility of bone marrow derived neural progenitor cells inducing microglia autophagy, and to identify the molecular mechanisms that might affect microglia growth through the identification of its metabolites and the isolation of exosomes. Methods The effects of exosomes on microglia were observed by cell biology and electron microscopy. The liposome metabolite types were identified by chemical reagent extraction and LCMS/MS detection technology. Results Molecular experiments have shown that this exosome can induce autophagy in microglia. The potential substances with the ratio of 4597 kinds of germplasm nuclei were detected in the culture medium of bone marrow-derived nerve progenitor cells, 304 kinds of known liposome metabolites were detected in the culture medium and bone marine-derived nerve progenitor cells. After excluding the inherent liposome metabolites in the culture medium, 51 kinds of potentially metabolized and secreted liposomes in the growth process of bone marrow derived nerve progenitor cells were obtained. These were all substances that can potentially cause autophagy in microglia. Conclusions Bone marrow derived nerve progenitor cells can induce autophagy in microglia and promote the initiation of immune response, during which liposome metabolites carried in exosomes of bone marrow derived nerve progenitor cells may play an important role.
Yuan Fengying, Zhang Mingxing, Shi Yihua, Li Meihui, Ou Jiayuan, Zhang Mingsheng , Bai Wenfang
. Research on the microglial cell autophagy induced by bone marrow stromal cells and its potential molecular mechanism [J]. Chinese Journal of Clinical Anatomy, 2021
, 39(3)
: 302
-310
.
DOI: 10.13418/j.issn.1001-165x.2021.03.011
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