Objective To investigate whether bone morphogenetic proteins 9 (BMP9) can activate PI3K/AKT signaling pathway to regulate osteogenic differentiation of human periodontal ligament fibroblasts (hPDLFs) under cyclic tension force. Methods The cultured in vitro hPDLFS were loaded with sine wave, deformation rate 10%, frequency 0.5 Hz periodic tension by FlexCell system. Distribution and changes of cytoskeletal protein expression were observed by immunofluorescence method. Changes of mRNA expression of RUNX2, OCN, OPN and OSX were observed by qPCR. Cytoskeletal protein OCN, OPN expressions and changes of AKT, P-AKT, OCN, OPN after adding P13K signal inhibitor LY294002 were detected by Western blot. Results Compared with control group, there were a significant increase of the expression of cytoskeletion-associated protein, up-regulation of the expression of osteogenic markers in 6 h and 12 h groups (P<0.05). The trend of mRNA expression was consistent with the result of protein detection. Conclusions Cyclic tension force-mediated BMP9 can regulate osteogenic differentiation of hPDLFs through PI3K/AKT signaling pathway.
ZHANG Chao, HU Jing, ZHANG Jun-yi, WEI Ke-yuan, SUN Hai-bao, HUANG Ke-qiang
. Cyclic tension force-mediated BMP9 regulates osteogenic differentiation of human periodontal ligament fibroblasts via PI3K/AKT signaling pathway[J]. Chinese Journal of Clinical Anatomy, 2019
, 37(6)
: 656
-661
.
DOI: 10.13418/j.issn.1001-165x.2019.06.010
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