Objective To experimentally investigate a potential mechanism of the TLR-NF-κB signaling pathway in the pathogenesis of allergic rhinitis (AR), and the role of NF-κB in eosinophil aggregation. Methods A total of 50 rats were randomly divided into five groups with A (control group), B (AR group), C (group of AR+PGN), D (group of AR+PGN+Pam3CSK4), and E (group of AR +PGN+BEL). AR model was established with ovalbumin. Hematoxylin and eosin (H&E) staining was used to detect the morphologic change of nasal mucosa and count the number of infiltrated inflammatory cell. The contents of IFN-γ, IL-4, MBP and IgE in nasal cleanser were detected by ELISA. Expression of NF-κB and TLR2 was measured by Real-Time polymerase chain reaction (PCR) and Western blot. Results Obvious allergic injury was observed in AR rats (B group). The number of infiltrated eosinophils in nasal mucosa in group B was higher than in other groups. The expressions of IL-4, MBP and IgE in group B were higher than in group A (P＜0.05). Neutrophil infiltration was predominant in nasal mucosa of group C, and the expression of IFN-γ, TLR2 and NF-κB was higher in group B (P＜0.05). Inflammatory cell infiltration, IL-4, MBP and IgE expression in group D and E were lower than in group B (P＜0.05). Conclusions Regulating the expression of TLR-NF-κB pathway and inhibiting the over-expression of NF-κB is beneficial to regulating the aggregation of eosinophils in the pathogenesis of allergic rhinitis.
ZHANG Min, WU Dan, LIU Li-xing, HOU Sai-rong, CAI Li-xi
. Role of NF-κB in pathogenesis of allergic rhinitis[J]. Chinese Journal of Clinical Anatomy, 2019
, 37(4)
: 421
-424
.
DOI: 10.13418/j.issn.1001-165x.2019.04.012
[1] Ren M, Tang Q, Chen F, et al. Mahuang fuzi xixin decoction attenuates Th1 and Th2 responses in the treatment of ovalbumin-induced allergic inflammation in a rat model of allergic rhinitis[J]. J Immunol Res, 2017, 8254324.
[2] Farrokhi S, Abbasirad N, Movahed A, et al. TLR9-based immunotherapy for the treatment of allergic diseases[J]. Immunotherapy, 2017, 9(4): 339-346.
[3] Sun B, Dwivedi N, Bechtel TJ, et al. Citrullination of NF-κB p65 promotes its nuclear localization and TLR-induced expression of IL-1β and TNFα[J]. Sci Immunol, 2017, 2(12). pii: eaal3062.
[4] 宋建明, 黄秀清, 林燕惠, 等. 变应性鼻炎大鼠模型的建立[J]. 吉林大学学报(医学版), 2012, 38(6): 1152-1155.
[5] Incorvaia C, Masieri S, Cavaliere C, et al. Asthma associated to rhinitis[J]. J Biol Regul Homeost Agents, 2018, 32(1 Suppl.1): 67-71.
[6] Corsico AG, De Amici M, Ronzoni V, et al. Allergen-specific immunoglobulin E and allergic rhinitis severity[J]. Allergy Rhinol(Providence), 2017, 8(1): 1-4.
[7] Aryan Z, Holgate ST, Radzioch D, et al. A new era of targeting the ancient gatekeepers of the immune system: toll-like agonists in the treatment of allergic rhinitis and asthma[J]. Int Arch Allergy Immunol, 2014, 164(1): 46-63.
[8] Sogut A, Yilmaz O, Kirmaz C. Regulatory-T, T-Helper 1, and T-Helper 2 cell differentiation in nasal mucosa of allergic rhinitis with olive pollen sensitivity[J]. Int Arch Allergy Immunol, 2012, 157(4): 349-353.
[9] Gour N, Wills-Karp M. IL-4 and IL-13 signaling in allergic airway disease[J]. Cytokine, 2015, 75(1): 68-78.
[10] Shin HS, Shon DH. Food and natural materials target mechanisms to effectively regulate allergic responses[J]. J Nutr Sci Vitaminol(Tokyo), 2015, 61 Suppl: S109-111.
[11] Carr W, Bernstein J, Lieberman P, et al. A novel intranasal therapy of azelastine with fluticasone for the treatment of allergic rhinitis[J]. J Allergy Clin Immunol, 2012, 129(5): 1282-1289.
[12] Gorelik M, Frischmeyer-Guerrerio PA. Innate and adaptive dendritic cell responses to immunotherapy[J]. Curr Opin Allergy Clin Immunol, 2015, 15(6): 575-580.
[13] Laiño J, Wangorsch A, Blanco F, et al. Targeting of immune cells by dual TLR2/7 ligands suppresses features of allergic Th2 immune responses in mice[J]. J Immunol Res, 2017, 7983217.
