Effects of different dosages of morphine on conditioned place preference in male C57 mice
XIE Min-Juan, LI Juan, ZHANG Lu
Chinese Journal of Clinical Anatomy ›› 2014, Vol. 32 ›› Issue (6) : 708-710.
Effects of different dosages of morphine on conditioned place preference in male C57 mice
Objective To observe the conditioned place preference (CPP) changes in mice after treated with different doses of morphine, and provide reference for the further study of morphine addiction. Method 3-month-old male C57 mice were injected intraperitoneally with 0 mg/kg, 5 mg/kg, 10 mg/kg, 15 mg/kg and 20 mg/kg of morphine, CPP and Straub tail reaction analysis were carried out. Result The Straub tail reaction analysis showed that: compared with control group, mice in morphine 10 mg/kg, 15 mg/kg, and 20 mg/kg groups demonstrated longer Straub tail reaction time (P< 0.05). The CPP analysis also showed that, compared with the control group mice，15mg/kg and 20mg/kg morphine reversed the natural tendency to black box of mice, which spent more time in the white box (P< 0.001). Meanwhile, the time spend in the white box was also statistically longer in the 10 mg/kg morphine group compared with the control group (P=0.049<0.05). Conclusion 15 mg/kg morphine can produce a significant adaptive change in CPP test in mice, which is an ideal dosage to construct morphine addiction model in mice.
C57 mice / Morphine / Dosage / Conditioned place preference / Straub tail reaction
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