Investigating the effects of different concentrations of estradiol on senescence model of human umbilical vein endothelial cells induced by hydrogen peroxide

doi:10.13418/j.issn.1001-165x.2019.05.010

Chinese Journal of Clinical Anatomy ›› 2019, Vol. 37 ›› Issue (5) : 528-533. DOI: 10.13418/j.issn.1001-165x.2019.05.010

LIN Jie-qi, RUAN Yun-jun, YANG Ru-yu, WANG Yu-yan, SU Shuang, WU Sai-zhu

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Abstract

Objective　To investigate the effects of different concentrations of estradiol on senescence model of human umbilical vein endothelial cells (HUVECs) induced by proper concentrations of hydrogen peroxide.　Methods　HUVECs were cultured and the senescence model was successful constructed induced by hydrogen peroxide, then the cultured cells were divided into the following five groups: a normal control, a senescence cells group, a low concentration 17β-E2 group, a physiological concentration 17β-E2 group and a high concentration 17β-E2 group. As results, we performed CCK-8 to detect cell viability, Western blot was conducted to evaluate the expression of the senescence related marker protein (p-Rb and Rb) and autophagocytosis related marker protein (P62 and LC3B), and the β-galactosidase staining also was tested to count the positive cells.　Results　As compared to the normal control, the senescence cells group had a higher protein expression value of p-Rb/Rb, decreased cells viability and more positive cells showed on β-galactosidase staining. On the contrary, in the physiological and high concentration groups, they not only had a lower protein expression value of p-Rb/Rb, increased cells viability, lesser positive cells showed on β-galactosidase staining than the senescence cells group but also had a decreased P62 protein expression and increased expression of LC3-Ⅱ/LC3-I protein which was closely related to autophagocytosis.　Conclusion 17β-E2 with concentration-dependent efficacy can inhibit HUVECs senescence induced by a proper concentrations of hydrogen peroxide through up-regulation of autophagocytosis.

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LIN Jie-qi, RUAN Yun-jun, YANG Ru-yu, WANG Yu-yan, SU Shuang, WU Sai-zhu. Investigating the effects of different concentrations of estradiol on senescence model of human umbilical vein endothelial cells induced by hydrogen peroxide[J]. Chinese Journal of Clinical Anatomy. 2019, 37(5): 528-533 https://doi.org/10.13418/j.issn.1001-165x.2019.05.010

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