Developing intervertebral disc degeneration at rabbit lumber spine by using low-temperature plasma radiofrequency ablation
ZHOU Wei, XU Xi-Chun, JIA Yin-Xing, WANG Ji-You, TU Dong-Jing, HUI Jia-Dong, TU Hao-Chao, DIAO Fei
Chinese Journal of Clinical Anatomy ›› 2013, Vol. 31 ›› Issue (6) : 696-701.
Developing intervertebral disc degeneration at rabbit lumber spine by using low-temperature plasma radiofrequency ablation
Objective To develop a new intervertebral disc(IVD) degeneration animal model by plasma radiofrequency ablation. Methods 24 New Zealand white rabbits were equally divided into the experimental group and control group. L3/4～L5/6 IVDs were punctured and ablated for 30 seconds in the experimental group and t were just punctured in the control group. DR imaging studies, MR imaging studies and histological analysis were performed prior to surgery and 4, 8, 12 weeks after surgery to monitor progression of IVDs degeneration. Results The DR and MR examinations showed degeneration signs like lost of IVD height and T2 density. The control group showed no significant changes in DR and MR scan after 4 weeks. Both groups showed no lost of nucleus pulposus in the early time points and the experimental group showed lost of nucleus pulposus and derangement of the IVD structure. Conclusions The practicable and reproducible animal IVD degeneration model proposed in this paper is more stable than the previous puncture model.
Low-temperature plasma radiofrequency ablation / Intervertebral disc degeneration / Animal model / Rabbit
[1] Singh K, Masuda K, An H. Animal models for human disc degeneration
[J].Spine J, 2005, 5（6）:267-279.
[2] Masuda K, Aota Y, Muehleman C, et al.A novel rabbit model of mild, reproducible disc degeneration by an anulus needle puncture: correlation between the degree of disc
injury and radiological and histological appearances of disc degeneration
[J]. Spine,2005,30(1):5-14.
[3] Pfirrmann C, Metzdorf A, Zanetti M, et al. Magnetic resonance classification of lumbar intervertebral disc degeneration
[J].Spine,2001,26(17):1873-1878.
[4] Cappello R, Bird J, Pfeiffer D,et al.Notochordal cell produce and assemble extracellular mMatrix in a distinct manner, which may be responsible for the maintenance of
healthy nucleus pulposus
[J].Spine,2006,31(8):873-882.
[5] Sobajima S, Kompel J, Kim J, et al. A slowly progressive and reproducible animal model of intervertebral disc degeneration characterized by MRI, X-Ray, and histology
[J].Spine,2005,30(1):15-24.
[6] Moon CH, Jacobs L, Kim JH,et al.Part 2: Quantitative proton T2 and sodium magnetic resonance imaging to assess intervertebral disc degeneration in a rabbit model
[J].Spine,2012,37(18):1113-1119.
[7] Zhang Y, Drapeau S, An HS,et al.Histological features of the degenerating interverterbral disc in a goat disc injury model
[J]Spine,2011,36(19):1519-27.
[8] Zhou RP, Zhang ZM, Wang L, et al.Establishing a disc degeneration model using computed tomography-guided percutaneous puncture techniquein the rabbit
[J].J Surg Res,2013
,181(2):65-74.
[9] Leckie SK, Bechara BP,Hartman RA, et al. Injection of AAV2-BMP2 and AAV2-TIMP1 into the nucleus pulposus slows the course of intervertebral discdegeneration in an in vivo
rabbit model
[J].Spine J,2012,12(1):7-20.
[10] Sowa G, Westrick E, Pacek C,et al. In vitro and in vivo testing of a novel regulatory system for gene therapy for intervertebral disc degeneration
[J]. Spine, 2011,36
(10):623-628.
[11] Hoogendoorn R, Helder M, Kroeze R,et al. Reproducible long-term disc degeneration in a large animal model
[J].Spine,2008,33(9):949-954.
[12]Zhang HN, Marca F, Hollister S, et al. Developing consistently reproducible intervertebral disc degeneration at rat caudal spine by using needle puncture
[J]. J Neurosurg
Spine,2009,10(6):522-530.
[13]Elliott DM，Yerramalli CS，Beckstein JC，et al. The effect of relative needle diameter in puncture and sham injection animal models of degeneration
[J]. Spine，2008，33（6）
：588-596.
[14]戎利民，张飞. 消融髓核成形术中消融时间与椎间盘内压变化关系的实验研究
[J].中国病理生理杂志，2008,24(10）：1975-1979.
[15]Gruber H,Johnson T,Norton J, et al. The sand rat model for disc degeneration: radiologic characterization of age-related changes
[J]. Spine，2002,27（3）：230-234.
[16]Hunter C, Matyas J, Duncan N. Cytomorphology of notochordal and chondrocytic cells from the nucleus pulposus: a species comparison
[J]. J Anat,2004,205(5):357-362.
/
| 〈 |
|
〉 |
